Characterization of representative rpoB gene mutations leading to a significant change in toyocamycin production of Streptomyces diastatochromogenes 1628

Journal of Industrial Microbiology & Biotechnology
Zheng MaXiaoping Yu

Abstract

Modification of enzymes involved in transcription- or translation-processes is an interesting way to increase secondary metabolite production in Streptomycetes. However, application of such methods has not been widely described for strains which produce nucleoside antibiotics. The nucleoside antibiotic toyocamycin (TM) is produced by Streptomyces diastatochromogenes 1628. For improving TM production in S. diastatochromogenes 1628, the strain was spread on rifamycin-resistant (Rif(r)) medium. Several spontaneous mutants were obtained with mutations in the rpoB gene which encodes a RNA polymerase β-subunit. The mutants which showed increased TM production were detected at a frequency of 7.5 % among the total Rif(r) mutants. Mutant 1628-T15 harboring amino acid substitution His437Arg was the best TM producer with a 4.5-fold increase in comparison to that of the wild-type strain. The worst producer was mutant 1628-T62 which also showed a poor sporulation behavior. RT-PCR was performed to study the transcription levels of the TM biosynthetic gene toyG in the parental strain as well as in mutants 1628-T15 and 1628-T62. The transcriptional level of toyG was higher in mutant 1628-T15 than that in parental strain 1628, while much lower ...Continue Reading

References

Nov 7, 2003·Applied and Environmental Microbiology·Norimasa TamehiroKozo Ochi
Nov 30, 2004·Advances in Applied Microbiology·Kozo OchiKazuhiko Kurosawa
Jan 5, 2006·Applied and Environmental Microbiology·Kazuhiko KurosawaKozo Ochi
Jun 26, 2007·Bioscience, Biotechnology, and Biochemistry·Kozo Ochi
Jan 22, 2010·FEMS Microbiology Reviews·Keith F ChaterHildgund Schrempf
Mar 23, 2010·Current Opinion in Microbiology·Juan-Francisco Martín, Paloma Liras
Jun 21, 2011·Applied and Environmental Microbiology·Maksym MyronovskyiAndriy Luzhetskyy
Sep 20, 2011·Journal of Bacteriology·Marcin WolanskiJolanta Zakrzewska-Czerwinska
Mar 28, 2012·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Akiyoshi HigoYasuo Ohnishi
Apr 23, 2013·Applied and Environmental Microbiology·Han-Na LeeEung-Soo Kim
Dec 21, 2013·Microbial Cell Factories·Héctor RodríguezRamón I Santamaría
Feb 22, 2014·Journal of Microbiological Methods·Divya Prakash, N N Nawani

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Citations

Mar 6, 2017·World Journal of Microbiology & Biotechnology·Shawn ChenSteven Van Lanen
Mar 30, 2020·Applied Microbiology and Biotechnology·Zhangqing SongXiaoping Yu
Jan 31, 2019·Journal of Industrial Microbiology & Biotechnology·Yanfang ZhaoXiaoping Yu
Sep 14, 2021·Journal of Zhejiang University. Science. B·Yefeng HuXiaoping Yu

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