PMID: 9174640Jan 1, 1997Paper

Characterization of retroviruses from patients with multiple sclerosis

Acta Neurologica Scandinavica. Supplementum
T ChristensenA Møller-Larsen

Abstract

These studies were performed to characterize retroviruses found in cell lines spontaneously developed from peripheral blood mononuclear cells (PBMNC) from 6 multiple sclerosis patients, a patient with progressive myelopathy and a healthy control. The cell lines are B-lymphoblastoid and produce Epstein-Barr virus (EBV) particles or express EBV proteins. The B-lymphoblastoid cell lines are also characterized by production of low, fluctuating amounts of retrovirus. The low productivity complicates purification and characterization, but implementation of product-enhanced reverse transcriptase (PERT) assays has provided a highly useful tool for monitoring retrovirus production. By electron microscopy, the retroviral particles appear type-C-like. Functional assays indicate the presence of Pol, Gag and Env. Indirect ELISA demonstrates a significant relation between disease activity and reactivity towards retroviral peptides. Molecular characterization is primarily based on RT-PCR, cloning, sequencing and Northern- or Southern analyses. Molecular characterization is continuing.

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Citations

Apr 28, 2010·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Tove Christensen
Oct 9, 2002·Journal of Virological Methods·Fumitomo OdawaraKouichi Sano
Oct 10, 1998·Journal of Virological Methods·A Møller-Larsen, T Christensen
Jan 27, 2012·Journal of Virology·Magdalena Janina LaskaBjørn Andersen Nexø
Mar 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·Cécile VoissetDavid J Griffiths
Jul 31, 2007·Future Microbiology·Sunit Kumar Singh
Jan 29, 2016·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Tove Christensen
Sep 22, 2005·Nature Reviews. Microbiology·Frederic BushmanChristian Hoffmann
Mar 17, 2001·Nature Reviews. Molecular Cell Biology·M Negroni, H Buc
Mar 2, 2010·Expert Review of Neurotherapeutics·Klaus Lauer
Jan 6, 1999·Acta Neurologica Scandinavica·M MunchS Haahr
Mar 6, 2003·Multiple Sclerosis : Clinical and Laboratory Research·T ChristensenA Møller-Larsen
Jul 27, 2018·Molecular Neurobiology·Gerwyn MorrisBasant K Puri

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