Characterization of rhamnolipids by liquid chromatography/mass spectrometry after solid-phase extraction

Analytical and Bioanalytical Chemistry
Beate BehrensHeiko Hayen

Abstract

Rhamnolipids are surface-active agents with a broad application potential that are produced in complex mixtures by bacteria of the genus Pseudomonas. Analysis from fermentation broth is often characterized by laborious sample preparation and requires hyphenated analytical techniques like liquid chromatography coupled to mass spectrometry (LC-MS) to obtain detailed information about sample composition. In this study, an analytical procedure based on chromatographic method development and characterization of rhamnolipid sample material by LC-MS as well as a comparison of two sample preparation methods, i.e., liquid-liquid extraction and solid-phase extraction, is presented. Efficient separation was achieved under reversed-phase conditions using a mixed propylphenyl and octadecylsilyl-modified silica gel stationary phase. LC-MS/MS analysis of a supernatant from Pseudomonas putida strain KT2440 pVLT33_rhlABC grown on glucose as sole carbon source and purified by solid-phase extraction revealed a total of 20 congeners of di-rhamnolipids, mono-rhamnolipids, and their biosynthetic precursors 3-(3-hydroxyalkanoyloxy)alkanoic acids (HAAs) with different carbon chain lengths from C8 to C14, including three rhamnolipids with uncommon C9 a...Continue Reading

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Oct 12, 2017·Applied Microbiology and Biotechnology·Jianwei ChenHong Wang
Dec 16, 2017·Microbial Cell Factories·Till TisoLars Mathias Blank
Mar 16, 2019·Water Environment Research : a Research Publication of the Water Environment Federation·Chul-Hwan KimJae-Jin Kim
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Jul 23, 2018·Applied Microbiology and Biotechnology·Andreas Wittgens, Frank Rosenau
Dec 19, 2016·Applied Microbiology and Biotechnology·Andreas WittgensFrank Rosenau
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Aug 17, 2021·Applied Microbiology and Biotechnology·Veronica SegoviaRegina Hernández-Gama

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