PMID: 9072524May 1, 1996Paper

Characterization of Rhizobium 'hedysari' by RFLP analysis of PCR amplified rDNA and by genomic PCR fingerprinting

The Journal of Applied Bacteriology
S Selenska-PobellA Squartini

Abstract

The taxonomic and discriminatory power of RFLP analysis of PCR amplified parts of rhizobial rrn operons was compared to those of genomic PCR fingerprinting with arbitrary and repetitive primers. For this purpose, the two methods were applied for characterization of a group of bacterial isolates referred to as Rhizobium 'hedysari'. As outgroups, representatives of the family Rhizobiaceae, belonging to the Rhizobium galegae, Rhizobium meliloti, Rhizobium leguminosarum and Agrobacterium tumefaciens species were used. By the RFLP analysis of the PCR products corresponding to the variable 5'-half of the 23S rRNA gene and of the amplified spacer region between the 16S and 23S rRNA genes all Rh. 'hedysari' strains studied were tightly clustered together while the outgroups were placed in an outer position. The PCR products of the 3' end parts of the 23S rDNA did not show significant RFL polymorphism and no species differentiation on their basis was possible. In parallel, analysis of the same strains was performed by PCR amplification of their DNA with 19, 18 and 10 bp long arbitrary primers (AP-PCR) as well as with single primers corresponding to several bacterial repetitive sequences (rep-PCR). By both AP and rep-PCR an identificatio...Continue Reading

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Citations

Feb 6, 2008·International Journal of Phytoremediation·R A I Abou-ShanabP van Berkum
Feb 19, 2002·Journal of Applied Microbiology·P QuatriniA M Puglia
Apr 3, 2003·FEMS Microbiology Letters·Emanuele G BiondiMarco Bazzicalupo
Apr 25, 2003·International Journal of Systematic and Evolutionary Microbiology·L KröckelW Ludwig

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