PMID: 9072524May 1, 1996Paper

Characterization of Rhizobium 'hedysari' by RFLP analysis of PCR amplified rDNA and by genomic PCR fingerprinting

The Journal of Applied Bacteriology
S Selenska-PobellA Squartini


The taxonomic and discriminatory power of RFLP analysis of PCR amplified parts of rhizobial rrn operons was compared to those of genomic PCR fingerprinting with arbitrary and repetitive primers. For this purpose, the two methods were applied for characterization of a group of bacterial isolates referred to as Rhizobium 'hedysari'. As outgroups, representatives of the family Rhizobiaceae, belonging to the Rhizobium galegae, Rhizobium meliloti, Rhizobium leguminosarum and Agrobacterium tumefaciens species were used. By the RFLP analysis of the PCR products corresponding to the variable 5'-half of the 23S rRNA gene and of the amplified spacer region between the 16S and 23S rRNA genes all Rh. 'hedysari' strains studied were tightly clustered together while the outgroups were placed in an outer position. The PCR products of the 3' end parts of the 23S rDNA did not show significant RFL polymorphism and no species differentiation on their basis was possible. In parallel, analysis of the same strains was performed by PCR amplification of their DNA with 19, 18 and 10 bp long arbitrary primers (AP-PCR) as well as with single primers corresponding to several bacterial repetitive sequences (rep-PCR). By both AP and rep-PCR an identificatio...Continue Reading


Jan 1, 1992·Journal of General Microbiology·D HsuL M Shih
Jan 1, 1987·Journal of Molecular Evolution·E GilsonM Hofnung
Sep 1, 1974·Journal of General Microbiology·J E Beringer
May 15, 1981·Journal of Molecular Biology·J BrosiusH F Noller
Jul 1, 1994·International Journal of Systematic Bacteriology·S M NourJ C Cleyet-Marel
Oct 1, 1995·The Journal of Applied Bacteriology·S Selenska-PobellN Petrova
Dec 1, 1995·Journal of Bacteriology·S Selenska-Pobell, E Evguenieva-Hackenberg
Oct 1, 1994·Letters in Applied Microbiology·M SanderyS McKersie-Donnolley
Nov 1, 1994·Letters in Applied Microbiology·H N RasmussenO F Rasmussen
May 1, 1994·Canadian Journal of Microbiology·S M NourM P Fernandez
Sep 1, 1994·The Journal of Applied Bacteriology·J M Farber, C J Addison
Jun 1, 1994·Applied and Environmental Microbiology·H ChristensenO F Rasmussen
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·D Ralph, M McClelland
Apr 1, 1993·International Journal of Systematic Bacteriology·A Willems, M D Collins
Dec 1, 1995·Letters in Applied Microbiology·E Evguenieva-Hackenberg, S Selenska-Pobell

❮ Previous
Next ❯


Feb 6, 2008·International Journal of Phytoremediation·R A I Abou-ShanabP van Berkum
Feb 19, 2002·Journal of Applied Microbiology·P QuatriniA M Puglia
Apr 3, 2003·FEMS Microbiology Letters·Emanuele G BiondiMarco Bazzicalupo
Apr 25, 2003·International Journal of Systematic and Evolutionary Microbiology·L KröckelW Ludwig

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.