PMID: 11605984Oct 19, 2001Paper

Characterization of Rhodococcus opacus R7, a strain able to degrade naphthalene and o-xylene isolated from a polycyclic aromatic hydrocarbon-contaminated soil

Research in Microbiology
P Di GennaroG Bestetti

Abstract

Rhodococcus opacus R7 was isolated from a soil contaminated with polycyclic aromatic hydrocarbons for its ability to grow on naphthalene. The strain was also able to degrade o-xylene, the isomer of xylenes most recalcitrant to microbial degradation. The catabolic pathways for naphthalene and o-xylene were investigated by identification of metabolites in R. opacus R7 cultures performed with the two hydrocarbons and by evaluation of some enzymes involved in the metabolism of these compounds. 1,2-Dihydro-1,2-dihydroxynaphthalene, salicylic and gentisic acids were identified as metabolites in cultures exposed to naphthalene. This suggests that the degradation occurs through the dioxygenation of the aromatic ring with the formation of 1,2-dihydro-1,2-dihydroxynaphthalene, dehydrogenated to the corresponding 1,2-dihydroxy derivative which is further oxidized to salicylic acid, a key intermediate of naphthalene metabolism; this compound is converted to gentisic acid cleaved by a gentisate 1,2-dioxygenase. From R. opacus R7 cultures supplied with o-xylene, 2,3-dimethylphenol and 3,4-dimethylcatechol were observed. The pathway of o-xylene involves the monooxygenation of the benzene nucleus leading to dimethylphenol which is further meta...Continue Reading

Associated Datasets

References

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Citations

Mar 3, 2010·Applied Microbiology and Biotechnology·Patrizia Di GennaroGiuseppina Bestetti
Jan 7, 2011·Applied Microbiology and Biotechnology·Xiao-Ying LuHerbert Han-Ping Fang
Oct 20, 2009·Applied and Environmental Microbiology·Marc AuffretFrançoise Fayolle-Guichard
Mar 7, 2003·Applied and Environmental Microbiology·Xue PengShigeaki Harayama
Mar 10, 2004·Applied and Environmental Microbiology·Daisuke IshiyamaJulian Davies
Dec 8, 2005·Applied and Environmental Microbiology·Takahiro MaruyamaNorihiko Misawa
Nov 9, 2007·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Rensheng ZhuangGyula Zaray
Nov 7, 2019·Environmental Science and Pollution Research International·Fatemeh GholamiMohammad Ali Amoozegar
Sep 10, 2020·Frontiers in Microbiology·Jessica ZampolliAlessandro Orro
Nov 18, 2018·The Science of the Total Environment·Klaudia KosekŻaneta Polkowska
Oct 31, 2021·Scientific Reports·Jessica ZampolliPatrizia Di Gennaro

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