PMID: 8995643Feb 1, 1997Paper

Characterization of ribonucleoprotein complexes containing an abundant polyadenylated nuclear RNA encoded by Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8)

Journal of Virology
W Zhong, D Ganem

Abstract

Infection with Kaposi's sarcoma-associated herpesvirus (KSHV) (also called human herpesvirus 8) is strongly linked to all forms of Kaposi's sarcoma. We have previously identified two polyadenylated KSHV transcripts that are actively transcribed in Kaposi's sarcoma (KS) tumors and in KSHV-infected B-lymphoma cells. One of these RNAs (termed T1.1 or nut-1 RNA) is a 1.1-kb transcript present in a subpopulation of KS tumor cells. This RNA is localized to the nucleus of infected cells and has no open reading frames longer than 62 codons, suggesting that it may not function as an mRNA in vivo. Here we demonstrate that nut-1 RNA is a lytic-cycle gene product that is found in high-molecular-weight ribonucleoprotein complexes in infected cell nuclei. The transcript lacks the trimethylguanosine (TMG) cap found in many U-like small nuclear RNAs, but a subpopulation of nut-1 RNAs can associate with Sm protein-containing small nuclear ribonucleoproteins, as judged by immunoprecipitation analyses using monoclonal anti-Sm and anti-TMG antibodies. This interaction does not require other viral gene products, and deletion of the sole candidate Sm binding site on nut-1 RNA does not ablate this association. This finding suggests an indirect intera...Continue Reading

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Citations

Feb 19, 2013·Journal of Medical Virology·Jair Carneiro LeaoStephen R Porter
Sep 24, 2002·Revista do Hospital das Clínicas·Jair Carneiro LeãoCrispian Scully
Aug 15, 2003·The Journal of General Virology·Brian P McSharryGavin W G Wilkinson
Dec 26, 2008·Journal of Virology·Anna R CliffeBernadette M Dutia
Mar 13, 2013·Expert Review of Molecular Diagnostics·Katrien Van RoosbroeckGeorge A Calin
May 13, 2003·Reviews in Medical Virology·Zhi-Ming Zheng
May 27, 2016·Journal of Virology·J Robert Hogg
Aug 26, 1998·Journal of the National Cancer Institute. Monographs·P S Moore, Y Chang
May 28, 2013·Briefings in Functional Genomics·Paulo P AmaralJohn S Mattick
Nov 20, 2016·Parasitology Research·Vanessa Gómez, Moisés Wasserman
Nov 7, 2014·Viruses·Mel CampbellYoshihiro Izumiya
Jun 17, 1998·Journal of Virology·C DiamondL Corey
Feb 11, 1999·Journal of Virology·R SunG Miller
Feb 22, 2002·International Journal of Cancer. Journal International Du Cancer·Rastine MeratAli Saib
Dec 13, 2000·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·J C LeaoC Scully
Jul 11, 1998·Oral Oncology·S R PorterN Kumar
Mar 12, 1998·Infectious Disease Clinics of North America·R M Greenblatt
Aug 5, 2009·Cancer Letters·Kwun Wah Wen, Blossom Damania

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