PMID: 38635Apr 1, 1979

Characterization of RNA polymerases from Rous sarcoma virus-induced mouse ascites sarcoma cells

Acta Medica Okayama
H Misumi, T Oda

Abstract

RNA polymerase was extracted from the Schmidt-Ruppin strain of Rous sarcoma virus (SR-RSV)-induced C3H/He mouse ascites sarcoma cells (SR-C3H). RNA polymerase was separated into RNA polymerases I and II by DEAE-Sephadex chromatography. RNA polymerase I was separated into Ia and Ib fractions by phospho-cellulose chromatography. In SR-C3H cells RNA polymerase Ib was the main component of RNA polymerase I. At 0.05--0.1 M ammonium sulphate RNA polymerase I transcribed native DNA most actively, and RNA polymerase II transcribed denatured DNA most actively. Partial digestion of DNA by DNAase I enhanced RNA synthesis by RNA polymerases I and II. At ionic strength over 0.2 M ammonium sulphate, the initiation reaction of RNA polymerases I and II was inhibited. The initiation complexes of RNA polymerases I and II with native DNA were more stable against high salt concentration than with denatured DNA.

Related Concepts

RNA Polymerase II
Cations, Divalent
RNA Denaturation
Transcription, Genetic
Transcriptase
Sarcoma, Avian
RNA Polymerase I
Genetic Template
Cell Transformation, Viral
Chicken Tumor 1 Virus

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