Characterization of RNASET2, the first human member of the Rh/T2/S family of glycoproteins

Archives of Biochemistry and Biophysics
Paola CampomenosiRoberto Taramelli

Abstract

Ribonucleases are ubiquitous enzymes involved in RNA metabolism and are classified in several families on the basis of their structural, catalytic, and biological properties. Here, we describe characterization of the only human member of the Rh/T2/S family of acid hydrolases so far described, named RNASET2. This protein was previously reported to have an interesting biological function in the control of tumourigenesis and metastatization. We show that RNASET2 is present in multiple forms in human cell lines and mouse tissues, one of which represents the full length, glycosylated and secreted form, while the others are proteolytic products. RNASET2 is endowed with catalytic activity as demonstrated with purified recombinant protein expressed in the Baculovirus Expression Vector System and in a human cell line ectopically expressing various types of constructs. Furthermore, we document for this protein a lysosomal localization as described for other members of the Rh/T2/S family of ribonucleases. The results presented herein represent a further advancement toward the molecular understanding of the tumour suppressive properties of the human RNASET2 protein.

References

Sep 1, 1995·Genes, Chromosomes & Cancer·L QueimadoS Castedo
Aug 26, 1998·Biochimica Et Biophysica Acta·R Le Borgne, B Hoflack
May 15, 2002·Genes, Chromosomes & Cancer·Eija-Riitta HyytinenJin-Tang Dong
Nov 15, 2003·Molecular Phylogenetics and Evolution·Eric H Roalson, Andrew G McCubbin
Jan 7, 2004·Nature Reviews. Molecular Cell Biology·Juan S Bonifacino
Jan 26, 2005·The Journal of Cell Biology·Valérie Laurent-MathaEmmanuelle Liaudet-Coopman

❮ Previous
Next ❯

Citations

Feb 26, 2010·Molecular Genetics and Genomics : MGG·Gustavo C MacIntoshLex Flagel
Jan 5, 2011·Proceedings of the National Academy of Sciences of the United States of America·Noémie HaudAdam F L Hurlstone
Jul 22, 2009·BMC Evolutionary Biology·Melissa S HillwigGustavo C MacIntosh
Jul 26, 2012·PloS One·Natalie Luhtala, Roy Parker
Feb 26, 2013·Molecular & Cellular Proteomics : MCP·Agnès ChapelAgnès Journet
Jun 13, 2015·The Journal of Investigative Dermatology. Symposium Proceedings·Qianqian WangLeihong Xiang
Jul 25, 2015·Cell Death and Differentiation·G CaputaJ E Schaffer
Mar 2, 2010·Trends in Biochemical Sciences·Natalie Luhtala, Roy Parker
Dec 23, 2011·Biology of the Cell·Laura VidalinoPaolo Macchi
Feb 14, 2012·The FEBS Journal·Marianna N KiritsiDiamantis C Sideris
Feb 11, 2015·Molecular Biotechnology·Marta LualdiFrancesco Acquati
Nov 4, 2008·Biochimica Et Biophysica Acta·Torben LübkeDavid E Sleat
Oct 26, 2018·Clinical Endocrinology·Aleksander KuśTomasz Bednarczuk
Sep 1, 2007·Proteomics. Clinical Applications·David E SleatPeter Lobel
Dec 4, 2014·The EMBO Journal·Hanghang HuangEiichiro Fukusaki
Jan 1, 2017·Journal of Biochemistry·Yuuki FujiwaraTomohiro Kabuta
Nov 22, 2019·Frontiers in Immunology·Francesco AcquatiDouglas M Noonan
Mar 28, 2018·Reproduction : the Official Journal of the Society for the Study of Fertility·Yali XuZhide Ding
Sep 10, 2020·Frontiers in Immunology·Lei WuYongsheng Li
Mar 27, 2020·Frontiers in Immunology·Nicolò BaranziniAnnalisa Grimaldi
May 8, 2020·Cells·Purvi C TrivediThomas Pulinilkunnil
Jun 20, 2020·Cell Chemical Biology·Irem Avcilar-KucukgozeAnna Kashina
Dec 24, 2020·International Journal of Molecular Sciences·Nicolò BaranziniAnnalisa Grimaldi
Jun 24, 2021·International Immunology·Kaiwen LiuKensuke Miyake
Oct 29, 2018·Journal of Innate Immunity·Nicolò BaranziniAnnalisa Grimaldi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.