PMID: 9431913Feb 12, 1998Paper

Characterization of rpoB mutations in rifampin-resistant clinical Mycobacterium tuberculosis isolates from Greece

Journal of Clinical Microbiology
P Matsiota-BernardE Marinis

Abstract

There is a geographic distribution of Mycobacterium tuberculosis strains with various rpoB gene mutations that account for rifampin resistance. We studied 17 rifampin-resistant clinical isolates from patients in Greece to identify rpoB mutations. The aim of our study was the evaluation of a commercially available line probe assay kit (INNO-LiPA Rif. TB) to detect rpoB mutations and rifampin resistance. The results obtained with the commercially available assay were compared to those obtained by automated DNA sequence analysis of amplified PCR products. Randomly amplified polymorphic DNA (RAPD) analyses of the isolates were also performed. The overall concordance of the line probe assay with phenotypic rifampin susceptibility test was 94%. Three distinct rpoB mutations in codons Ser531, His526, and Asp516 were correctly identified with the kit, but mutations in external regions and insertions were detected only by automated DNA sequence analysis. The changes in codons Ser531 and His526 accounted for the majority of rifampin resistance, as previously described for isolates from other geographic areas. The results obtained by RAPD analyses of the isolates suggested that clonally related M. tuberculosis strains can have subclones b...Continue Reading

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Citations

Aug 7, 2020·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Nolwazi L Bhembe, Ezekiel Green
Apr 23, 2003·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·José María Viader-SalvadóMartha Guerrero-Olazarán
Jan 15, 2005·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Zeynep Ceren KarahanNejat Akar
Dec 21, 2006·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·E Tortoli, F Marcelli
Mar 7, 2003·Journal of Medical Microbiology·Hsing-Yu HwangYi-Jing Chen
Apr 4, 2001·Memórias do Instituto Oswaldo Cruz·S Spindola de MirandaE Drouet
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Jun 27, 2001·Applied and Environmental Microbiology·L Miché, J Balandreau
Dec 19, 2001·Emerging Infectious Diseases·G E PfyfferJ D van Embden
Jun 27, 2012·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Chamila Priyangani AdikaramSandhya Sulochana Wijesundera
Dec 20, 2014·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Parviz MohajeriSara Atashi
Jul 12, 2017·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Lamprini GkaravelaIris Spiliopoulou
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