Abstract
A cDNA, designated as rtSH3p13, was isolated from a rat testis cDNA library. It consists of 1463 bp nuclear acids, which encodes a protein of 312 amino acids and was assigned the GenBank accession number AF227439. The deduced rtSH3p13 protein is a truncated isoform of SH3p13 as a result of mRNA alternative splicing. It is mainly expressed in the rat testis, detected in spermatids at the steps 8-19 of spermiogenesis, and found around the acrosome. During postnatal development, rtSH3p13 appears on day 18 and reaches maximum on day 60. Further experimental results suggested that rtSH3p13 forms a complex with activated epidermal growth factor receptor (EGFR) and interacts with synaptojanin I. Surprisingly, similar to SH3 domain, the V region of rtSH3p13 also inhibits endocytosis in CHO cells. Our results reveal a link between an rtSH3p13-synaptojanin-clathrin complex-mediated formation of pits and the process of spermiogenesis.
References
Sep 1, 1990·Biology of Reproduction·R A Hess
Jan 1, 1989·Biology of the Cell·D Segretain
Jan 1, 1972·Physiological Reviews·Y Clermont
Mar 28, 1997·The Journal of Biological Chemistry·E de HeuvelP S McPherson
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·C W SoL C Chan
May 1, 1997·Genomics·C GiachinoN Migone
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·N RingstadP De Camilli
Oct 7, 1997·FEBS Letters·K D MichevaP S McPherson
Oct 27, 1997·The Journal of Biological Chemistry·K D MichevaP S McPherson
Jun 1, 1996·Nature Biotechnology·A B SparksB K Kay
Apr 7, 1999·Genomics·T FujiiY Abiko
Jun 1, 1999·Anatomy and Embryology·K Steger
Sep 18, 1999·Nature·A SchmidtH D Söling
Oct 27, 1999·Cell·O CremonaP De Camilli
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y TangR J Lefkowitz
Nov 13, 1999·Nature Cell Biology·F SimpsonS L Schmid
Oct 14, 2000·Annual Review of Cell and Developmental Biology·J E Hinshaw
Oct 26, 2000·Nature·J TranS DiNardo
Mar 22, 2001·Nature Reviews. Neuroscience·V I Slepnev, P De Camilli
Jul 17, 2001·Current Opinion in Cell Biology·N Jarousse, R B Kelly
Aug 24, 2001·The Journal of Biological Chemistry·N RingstadP De Camilli
Oct 18, 2001·The Journal of Cell Biology·K FarsadP De Camilli
Mar 15, 2002·Nature·Philippe SoubeyranIvan Dikic
Apr 17, 2002·Cell·Patrik VerstrekenHugo J Bellen
Mar 1, 1957·The American Journal of Anatomy·Y CLERMONT, B PEREY
Citations
Dec 25, 2010·Cell Biochemistry and Biophysics·Ole KjaerulffAnita Jung
Oct 29, 2010·Molecular Biology of the Cell·Ning ZhangLinfang Wang
Apr 11, 2014·PloS One·Shan LuWei Song
Jun 8, 2012·FEBS Letters·Xiaowen ChengLinfang Wang
May 22, 2010·FEBS Letters·Hongyu WuLinfang Wang
Jun 24, 2009·European Journal of Cell Biology·Shuchun LiLingfang Wang
Aug 27, 2021·The Biochemical Journal·Katherine M Wood, Corinne J Smith