Characterization of Schizosaccharomyces pombe mcm7(+) and cdc23(+) (MCM10) and interactions with replication checkpoints.

Genetics
D T Liang, S L Forsburg

Abstract

MCM proteins are required for the proper regulation of DNA replication. We cloned fission yeast mcm7(+) and showed it is essential for viability; spores lacking mcm7(+) begin S phase later than wild-type cells and arrest with an apparent 2C DNA content. We isolated a novel temperature-sensitive allele, mcm7-98, and also characterized two temperature-sensitive alleles of the fission yeast homolog of MCM10, cdc23(+). mcm7-98 and both cdc23ts alleles arrest with damaged chromosomes and an S phase delay. We find that mcm7-98 is synthetically lethal with the other mcmts mutants but does not interact genetically with either cdc23ts allele. However, cdc23-M36 interacts with mcm4ts. Unlike other mcm mutants or cdc23, mcm7-98 is synthetically lethal with checkpoint mutants Deltacds1, Deltachk1, or Deltarad3, suggesting chromosomal defects even at permissive temperature. Mcm7p is a nuclear protein throughout the cell cycle, and its localization is dependent on the other MCM proteins. Our data suggest that the Mcm3p-Mcm5p dimer interacts with the Mcm4p-Mcm6p-Mcm7p core complex through Mcm7p.

References

Jul 23, 1976·Molecular & General Genetics : MGG·P NurseK Nasmyth
Jan 1, 1991·Methods in Enzymology·S MorenoP Nurse
Oct 1, 1994·Molecular Biology of the Cell·K TakahashiM Yanagida
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·H ArakiA Sugino
Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·P RomanowskiR A Laskey
Jun 3, 1997·Gene·S L Forsburg, D A Sherman
Sep 26, 1997·The Journal of Biological Chemistry·Y Ishimi
Jul 1, 1997·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Y AdachiM Yanagida
Jan 1, 1997·Annual Review of Cell and Developmental Biology·A Dutta, S P Bell
May 9, 1998·The Journal of Biological Chemistry·Y IshimiH Kimura
May 23, 1998·Science·M N BoddyP Russell
Aug 5, 1998·Biochimica Et Biophysica Acta·S E Kearsey, K Labib
Aug 7, 1998·Molecular Biology of the Cell·D Bhaumik, T S Wang
Aug 15, 1998·The Journal of Biological Chemistry·G W Brown, T J Kelly
Sep 22, 1999·Molecular and Cellular Biology·Y OgawaH Masukata
Dec 10, 1999·Molecular Biology of the Cell·S G Pasion, S L Forsburg
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·Z KelmanJ Hurwitz
Jan 25, 2000·The Journal of Biological Chemistry·T A Prokhorova, J J Blow
Mar 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·J P ChongB Stillman
Jun 17, 2000·Trends in Cell Biology·M J O'ConnellA M Carr
Jun 29, 2000·Annual Review of Biochemistry·B K Tye

❮ Previous
Next ❯

Citations

Jan 30, 2002·Yeast
Oct 21, 2004·Molecular Cell·Robin M Ricke, Anja-Katrin Bielinsky
May 25, 2006·The Journal of Biological Chemistry·Karen Fien, Jerard Hurwitz
Jan 23, 2008·Biochemical Society Transactions·Susan L Forsburg
Jun 20, 2006·Molecular and Cellular Biology·Sapna Das-BradooAnja-Katrin Bielinsky
Jul 8, 2003·The Journal of Biological Chemistry·Craig R CookMing Lei
Feb 10, 2004·The Journal of Biological Chemistry·Karen FienJerard Hurwitz
Mar 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Susan L Forsburg
Sep 16, 2003·Molecular Biology of the Cell·Juraj GreganStephen E Kearsey
Mar 5, 2003·Journal of Molecular Biology·Margot KneisslFriedrich Grummt
Sep 24, 2010·BMC Biochemistry·Xiaoli MaMegan J Davey
Aug 5, 2011·The Journal of Biological Chemistry·Lin Ding, Susan L Forsburg
Dec 17, 2008·Structure·Eric M WarrenBrandt F Eichman
Jul 13, 2004·RNA·Sergei V ReverdattoDmitry A Belostotsky

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.