Characterization of senescence-associated proteases in postharvest broccoli florets

Plant Physiology and Biochemistry : PPB
Yuh Tai WangJei-Fu Shaw

Abstract

We characterized the senescence-associated proteases of postharvest broccoli (Brassica oleracea L. var Green King) florets, using class-specific protease inhibitors and gelatin-polyacrylamide gel electrophoresis. Different classes of senescence-associated proteases in broccoli florets were partially characterized for the first time. Protease activity of broccoli florets was depressed by all the inhibitors and showed different inhibition curves during postharvest. The hydrolytic activity of metalloprotease (EC 3.4.24. - ) and serine protease (EC 3.4.21. - ) reached a maximum, 1 day after harvest (DAH), then decreased, while the hydrolytic activity of cysteine protease (EC 3.4.22. - ) and aspartic protease (EC 3.4.23. - ) increased throughout the postharvest senescence based on the calculated inhibition percentage of protease activity. The senescence-associated proteases were separated into seven endoprotease (EP) groups by gelatin-polyacryamide gel electrophoresis and classified into EP1 (metalloprotease), EP2 (metalloprotease and cysteine protease), EP3 (serine protease and aspartic protease), EP4, EP5, EP7 (cysteine protease), and EP6 (serine protease) based on the sensitivity of class-specific protease inhibitors. The proteas...Continue Reading

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Citations

Jul 12, 2005·Plant Physiology and Biochemistry : PPB·Cecília M Antão, F Xavier Malcata
Aug 24, 2010·Journal of Plant Physiology·Agustin M BüchertGustavo A Martínez
Mar 9, 2010·Ecotoxicology and Environmental Safety·Ewa Gajewska, Maria Skłodowska
Sep 19, 2012·The New Phytologist·I D'ErfurthK Gallardo
Nov 8, 2014·Biotechnology Progress·Ilaria BenucciKatia Liburdi

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