Characterization of serum small extracellular vesicles and their small RNA contents across humans, rats, and mice.

Scientific Reports
Fengbo ZhaoJuling Ji

Abstract

Serum small extracellular vesicles (sEVs) have recently drawn considerable interest because of the diagnostic and therapeutic potential of their miRNAs content. However, the characteristics of human, mouse and rat serum sEVs and their differences in small RNA contents are still unknown. In this study, through nanoparticle tracking analysis and small RNA sequencing, we found that human, rat, and mouse serum sEVs exhibited distinct sizes and particle numbers as well as small RNA contents. Serum sEVs contained not only abundant miRNAs but also a large number of tRNA fragments. Most serum miRNAs existed both inside and outside of sEVs but were enriched in sEVs. Common serum sEV miRNAs (188 miRNAs) and species-specific serum sEV miRNAs (265, 58, and 159 miRNAs, respectively) were identified in humans, rats, or mice. The serum sEVs contained miRNAs from tissues and organs throughout the body, with blood cells as the main contributors. In conclusion, our findings confirmed the rationality of exploring serum sEV miRNAs as noninvasive diagnostic markers and revealed great differences in serum sEV small RNAs between humans, rats, and mice. Inadequate attention to these differences and the contribution of blood cells to serum sEV miRNAs c...Continue Reading

References

Apr 28, 2005·The Journal of Experimental Biology·John R Speakman
Jan 30, 2008·Current Protocols in Cell Biology·Clotilde ThéryAled Clayton
Jul 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Patrick S MitchellMuneesh Tewari
Mar 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jason D ArroyoMuneesh Tewari
Jun 12, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Shashi BalaGyongyi Szabo
Jan 9, 2013·Proceedings of the National Academy of Sciences of the United States of America·Roy L MauteRiccardo Dalla-Favera
Feb 20, 2013·The Journal of Cell Biology·Graça Raposo, Willem Stoorvogel
Mar 12, 2013·Nature·Toshikatsu HanadaJosef M Penninger
Sep 7, 2013·Journal of Extracellular Vesicles·Kenneth W WitwerFred Hochberg
Jan 1, 2012·Genes·Maria Rosa Garcia-SilvaAlfonso Cayota
Sep 16, 2014·FEBS Letters·Paul Anderson, Pavel Ivanov
Nov 14, 2014·Nucleic Acids Research·Pankaj KumarAnindya Dutta
Jan 9, 2015·Scientific Reports·Sara R SelitskyPraveen Sethupathy
Jan 1, 2014·Scientific Data·Keiichi MinamiTetsuro Urushidani
Sep 16, 2015·Scientific Reports·Xiaoming JiChunhui Ni
Oct 10, 2015·Translational Stroke Research·Fan ChenXunming Ji
Mar 21, 2017·The Journal of Clinical Investigation·Prashanth VallabhajosyulaAli Naji
May 17, 2017·Nature Communications·Akiko TakahashiEiji Hara
Jun 22, 2017·Nature Reviews. Gastroenterology & Hepatology·Gyongyi Szabo, Fatemeh Momen-Heravi
Dec 19, 2017·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Banishree SahaGyongyi Szabo
Jun 12, 2018·Journal of Extracellular Vesicles·Dominik BuschmannMarlene Reithmair
Aug 8, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jinjing Cun, Qifeng Yang

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Citations

Jul 9, 2020·American Journal of Physiology. Cell Physiology·Douglas W Van PeltEsther E Dupont-Versteegden
Jun 4, 2021·Arteriosclerosis, Thrombosis, and Vascular Biology·Laura R Goldberg

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Methods Mentioned

BETA
transmission electron microscopy
chips
PCR
RNA-seq
RNAseq
electron microscopy
electrophoresis

Software Mentioned

GraphPad Prism Prism
MISEV
Blastn
Cutadapt
FASTX Toolkit
Blast
Rfam

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