Characterization of serum tartrate-resistant acid phosphatase and development of a direct two-site immunoassay

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
Jussi M HalleenH Kalervo Väänänen

Abstract

Osteoclasts secrete tartrate-resistant acid phosphatase (TRAP) to the circulation, where the amount of TRAP is expected to correlate with the bone resorption rate. We have developed two monoclonal antibodies, O1A and J1B, using purified human bone TRAP as antigen. The antibodies recognized different epitopes, allowing us to develop a two-site fluoroimmunoassay. The immunoreactivity in fresh serum specimens was less than 10% of the concentrations measured from the same specimens after 24 h of storage at 4 degrees C, or after addition of 5 mM EDTA or EGTA to them. When fresh serum was gel filtrated using Sephacryl S-200 column, all of the enzyme eluted in the void volume as a complex with a molecular weight of more than 250 kDa. If the serum was treated with EDTA before the gel filtration, the complex was destroyed and the enzyme eluted in fractions corresponding to a molecular weight of 30 kDa, the size of monomeric purified human bone TRAP. The immunoassay was used to measure TRAP concentrations from serum samples that had been stored at 4 degrees C for 24 h. According to the assay, premenopausal women had 13.1 +/- 3.1, postmenopausal women 17.6 +/- 4.2, and children 32.6 +/- 12.2 microg TRAP/l of serum. We conclude that TRAP c...Continue Reading

References

Aug 1, 1990·The Journal of Clinical Endocrinology and Metabolism·M E KraenzlinD J Baylink
Jan 1, 1985·Enzyme·T Efstratiadis, D W Moss
Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J C Davis, B A Averill
Mar 1, 1984·Analytical Biochemistry·I HemmiläT Lövgren
Mar 1, 1996·The Histochemical Journal·A J JanckilaL T Yam
Aug 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J HellmanK Pettersson
Oct 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J HalleenH K Väänänen
Apr 11, 1997·Science·S A Nesbitt, M A Horton
Apr 11, 1997·Science·J SaloH K Väänänen

❮ Previous
Next ❯

Citations

Feb 5, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C S BrehmeR Wolfert
Jul 14, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J M HalleenH K Väänänen
Feb 23, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J M HalleenK Pettersson
Nov 17, 2009·Calcified Tissue International·Anthony J Janckila, Lung T Yam
Feb 19, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Shuichi MiyazakiTsugikazu Komoda
Apr 25, 2007·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·M G SørensenM A Karsdal
Jun 16, 2007·Biochemical and Biophysical Research Communications·Pirkko MuhonenH Kalervo Väänänen
Feb 19, 2002·Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine·U WeileR Claus
May 19, 2007·Chemistry & Biodiversity·Pirkko MuhonenTiina Laitala-Leinonen
Nov 7, 2003·The Journal of Clinical Endocrinology and Metabolism·Berthold FohrMarkus J Seibel
May 19, 2011·Toxicology and Applied Pharmacology·Mirja NurmioTiina Laitala-Leinonen
Jun 22, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·Y IgarashiS Matsuzaki
Oct 6, 2000·Clinica Chimica Acta; International Journal of Clinical Chemistry·K TakahashiL T Yam
Jun 3, 1999·Kidney International·P Ureña, M C De Vernejoul
Nov 27, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yoshihiko IgarashiShigeru Matsuzaki
Aug 7, 2003·Biochemical and Biophysical Research Communications·Hannele YlipahkalaH Kalervo Väänänen
Oct 19, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Tatsuya OhashiFumio Nomura
Jul 27, 2011·Journal of Immunological Methods·Silvia D Potenziani PradellaAnthony J Janckila

❮ Previous
Next ❯

Related Concepts

Related Feeds

Related Papers

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
Jussi M HalleenKim Pettersson
Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
Jussi M HalleenH Kalervo Väänänen
Clinica Chimica Acta; International Journal of Clinical Chemistry
Shuichi MiyazakiTsugikazu Komoda
Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
C S BrehmeR Wolfert
© 2022 Meta ULC. All rights reserved