Characterization of Shigella type 1 fimbriae: expression, FimA sequence, and phase variation.

Infection and Immunity
N J SnellingsM M Venkatesan

Abstract

This study documents the presence of type 1 fimbriae on Shigella and confirms these mannose-sensitive adherence structures to be bona fide components of the Shigella surface. While laboratory-passaged Shigella strains and lyophilized clinical isolates failed to express type 1 fimbriae, 6 of 20 recent clinical isolates, including 4 Shigella flexneri strains, 1 Shigella boydii strain, and 1 Shigella dysenteriae strain, produced type 1 fimbriae as detected by mannose-sensitive hemagglutination (MSHA) and electron microscopy. Optimal production of a predominantly Fim+ population required serial passage every 48 to 72 h in unshaken brain heart infusion broth at 37 degrees C. Fim+ Shigella cultures were capable of reversibly switching to a non-MSHA, afimbriated phase during serial aerobic cultivation on tryptic soy agar plates. The amino acid sequence of S. flexneri type 1 FimA contained 18 substitutions compared to that of Escherichia coli fimbrillin. Indirect immunoelectron microscopy suggested the presence of both shared and unique epitopes on E. coli and S. flexneri type 1 fimbriae. Random phase variation between fimbriated and afimbriated states in Shigella was accompanied by the genomic rearrangement associated with phase varia...Continue Reading

References

Jul 1, 1975·The Journal of Infectious Diseases·M M RahamanA K Kibriya
Jan 1, 1975·Annual Review of Microbiology·J C Ottow
Mar 1, 1992·Molecular Microbiology·C A BlochP E Orndorff
Jan 1, 1992·Microbiology and Immunology·A UtsunomiyaA Hamamoto
Jan 1, 1991·Clinical Microbiology Reviews·J R Johnson
Jun 1, 1991·Microbiological Reviews·T L Hale
May 1, 1985·Journal of Bacteriology·K BuchananS I Hull
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J M AbrahamB I Eisenstein
Aug 1, 1984·Journal of Bacteriology·P E Orndorff, S Falkow
Jun 1, 1993·The Journal of Veterinary Medical Science·T SekizakiI Nonomura

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Citations

May 23, 2012·Applied Microbiology and Biotechnology·Anja Ganner, Gerd Schatzmayr
May 19, 2000·Infection and Immunity·H SakellarisB Adler
Aug 15, 2000·Microbiology and Immunology·A UtsunomiyaA Hamamoto
Sep 14, 2007·Journal of Applied Microbiology·P M BeckerP G van Wikselaar
Apr 13, 2006·World Journal of Gastroenterology : WJG·Greg HarrisWangxue Chen
Nov 15, 2019·MSphere·Rachael B ChaninChristina S Faherty
Aug 26, 1998·Trends in Microbiology·R A Edwards, J L Puente

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