PMID: 12763136May 24, 2003Paper

Characterization of Siglec-5 (CD170) expression and functional activity of anti-Siglec-5 antibodies on human phagocytes

Experimental Hematology
Connie L Erickson-MillerAndrew G King

Abstract

The Siglec family of proteins consists of at least 10 members with immunoglobulin and lectin domains and with similar sialic acid-binding properties. Many Siglec family members are expressed on hematopoietic cells and are involved in cell/cell interactions. Some family members are suspected of regulating cellular processes through specific signaling pathways. Monoclonal antibodies were generated against specific epitopes of Siglec-5 (CD170) and were used to determine expression of Siglec-5 on normal blood and marrow cells and cell lines. The antibodies also were used to elucidate functional activity for Siglec-5 on blood neutrophils. Flow cytometry and ELISA were used to determine the specificity of the monoclonal antibodies for Siglec-5 and to determine expression patterns. Chemiluminescence assays were employed to measure the oxidative burst activity of whole blood or purified neutrophils following treatment with the anti-Siglec-5 antibodies. Cell surface expression analysis demonstrated that the protein was expressed on gated human neutrophil and monocyte populations, both in the blood and bone marrow. Expression on neutrophils was enhanced by one-hour treatment with fMLP or TNF-alpha. Epitope-specific anti-Siglec-5 monoclon...Continue Reading

References

Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·D SgroiI Stamenkovic
Feb 1, 1997·Current Biology : CB·L NitschkeM C Lamers
Jan 1, 1997·Annual Review of Immunology·T F TedderJ H Kehrl
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·S SatoT F Tedder
Jan 16, 1999·The Journal of Biological Chemistry·J BlasioliM L Thomas
Jan 8, 2000·The Journal of Biological Chemistry·H FloydP R Crocker
Jun 20, 2001·Immunology·P R Crocker, A Varki
Oct 24, 2001·Current Opinion in Oncology·E L Sievers, M Linenberger

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Citations

Nov 19, 2004·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Jun 16, 2005·Current Opinion in Pharmacology·Paul R Crocker
Mar 24, 2007·Nature Reviews. Immunology·Paul R CrockerAjit Varki
Jun 23, 2009·Pediatric Research·Ramachandran RashmiJoyce M Koenig
Jan 3, 2014·Developmental and Comparative Immunology·Z EscalonaJ Domínguez
Aug 22, 2009·American Journal of Reproductive Immunology : AJRI·Kota HattaB Anne Croy
Dec 17, 2008·Annals of the New York Academy of Sciences·Stephan von Gunten, Bruce S Bochner
Feb 23, 2012·Annals of the New York Academy of Sciences·Paul R CrockerHannah E Richards
Jan 28, 2015·Protein Expression and Purification·Johannes M PröpsterMario Schubert
Nov 29, 2016·Scientific Reports·Marion PepinCécile V Denis
Aug 5, 2004·The Journal of Biological Chemistry·Yuzuru IkeharaJames C Paulson
Jul 15, 2005·Glycobiology·Ajit Varki, Takashi Angata
Jun 24, 2008·Journal of Receptor and Signal Transduction Research·Sem GeniniElisabetta Giuffra
Jan 10, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yuxi ZhaoAlex J McCarthy
Aug 7, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Peter GunnarssonMagnus Grenegård

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