Characterization of Skn-1a/i POU domain factors and linkage to papillomavirus gene expression.

The Journal of Biological Chemistry
B AndersenM G Rosenfeld

Abstract

Tissue-restricted POU domain transcription factors, which bind octamer or octamer-like gene sequences, play roles in cellular differentiation and the development of several organs. We have previously identified a POU domain gene, Skn-1a/i, expressed primarily in epidermis, that encodes at least two products through alternative splicing. One of these, Skn-1a, acts as a transcriptional activator, and the other, Skn-1i, contains an inhibitory domain in the NH2 terminus, which prevents DNA-binding in vitro and transcriptional activation in vivo. We now demonstrate that when Skn-1i is expressed in eukaryotic cells it can bind to an octamer site, suggesting that in vivo cellular factors modulate the activity of the inhibitory domain to permit DNA-binding. Yet the inhibitory domain does not allow transactivation by Skn-1i or by a heterologous transactivator containing this domain in cis. Furthermore, we demonstrate that Skn-1a, Tst-1, and Oct-1 are the major octamer-binding proteins in epidermis. Since Skn-1a is primarily expressed in suprabasal cells of the epidermis, we have tested its possible role in the regulation of epidermal papillomaviruses. In transient transfection assays, Skn-1a and Tst-1 can activate the long control regio...Continue Reading

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Citations

Jan 19, 1999·Dermatologic Clinics·N A Saunders, I H Frazer
Aug 28, 2002·Journal of the European Academy of Dermatology and Venereology : JEADV·G Sanclemente, D K Gill
May 2, 2006·Stem Cells and Development·Netta R BlondheimDaniel Offen
Oct 5, 2010·Biochemical and Biophysical Research Communications·Anna Maria LenaEleonora Candi
Jan 20, 2005·Gene·Amber KahleMark A Nelson
Oct 26, 2016·Veterinary and Comparative Oncology·E SemikM Bugno-Poniewierska
Sep 3, 1999·The Journal of Biological Chemistry·J HildesheimJ C Vogel
Mar 8, 2003·The Journal of Biological Chemistry·Adriana CabralClaude Backendorf

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