Characterization of sodium-calcium exchange in rabbit renal arterioles

Kidney International
B C FowlerP D Bell

Abstract

Experiments were performed to test the hypothesis that renal arterioles exhibit Na-Ca exchange capability and that this process is regulated by protein kinase C (PKC). Glomeruli with attached arterioles were dissected from rabbit kidney and loaded with fura-2 for measurement of intracellular calcium concentration ([Ca2+]i) using microscope-based photometry. In tissue bathed in Ringer's solution containing 150 mM Na+ and 1.5 mM Ca2+, afferent and efferent arteriolar [Ca2+]i averaged 136 +/- 6 and 154 +/- 7 nM, respectively. Removal of extracellular Na+ increased afferent arteriolar [Ca2+]i by 70 +/- 7 mM, while efferent arteriolar [Ca2+]i only increased by 39 +/- 5 nM (P < 0.01 vs. afferent arteriole). These responses were inhibited by 6 nM Ni2+ and required extracellular Ca2+, but were unaffected by 10 microM diltiazem. After incubation in 500 microM ouabain, 5 microM monensin, and 5 microM nigericin, [Ca2+]i responses to removal of extracellular Na+ were exaggerated significantly, averaging 174 +/- 50 nM in afferent arterioles and 222 +/- 82 nM in efferent arterioles (NS vs. afferent arterioles). Moreover, responses to removal of extracellular Na+ were enhanced by 100 nM phorbol 12-myristate 13-acetate, an affect which was blo...Continue Reading

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Citations

Feb 26, 2000·Acta Physiologica Scandinavica·P D BellM T Unlap
Sep 13, 2003·Kidney International·Yilin RenJeffrey L Garvin
Jul 1, 1997·Clinical and Experimental Pharmacology & Physiology·P D Bell, J Y Lapointe
Feb 12, 2009·Clinical and Experimental Pharmacology & Physiology·Shin SomaTomohiko Kimura
Apr 21, 2005·Kidney International·Ruisheng LiuJeffrey L Garvin
Feb 20, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Bharathy Ponnuchamy, Raouf A Khalil
May 23, 2008·American Journal of Physiology. Renal Physiology·Xuemei WangRodger Loutzenhiser
Mar 7, 2008·Kidney & Blood Pressure Research·Julia Laske-ErnstUlrich Russ
Nov 21, 2014·Clinical and Experimental Nephrology·Midori Sasaki YatabeTsuyoshi Watanabe
Aug 30, 2011·Pflügers Archiv : European journal of physiology·Charlotte Mehlin SorensenMax Salomonsson
Apr 3, 2015·Physiological Reviews·Mattias CarlströmWilliam J Arendshorst
Jul 20, 2012·American Journal of Physiology. Renal Physiology·Charlotte Mehlin SorensenNiels-Henrik Holstein-Rathlou
Jul 15, 2000·American Journal of Physiology. Renal Physiology·M T UnlapP D Bell
Jul 3, 1999·Physiological Reviews·M P Blaustein, W J Lederer
Jan 3, 2001·American Journal of Physiology. Renal Physiology·F SchwedaA Kurtz
Jan 16, 2003·American Journal of Physiology. Renal Physiology·E F HwangP D Bell
Apr 13, 1999·The American Journal of Physiology·N A MashburnP D Bell
Apr 13, 1999·The American Journal of Physiology·L D NelsonP D Bell
Oct 12, 1999·The American Journal of Physiology·S K Fellner, W J Arendshorst

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