Characterization of soluble HLA molecules in sweat and quantitative HLA differences in serum of healthy individuals

Journal of Immunogenetics
N ZavazavaW Müller-Ruchholtz

Abstract

Soluble class I molecules were immunoprecipitated from human sweat and serum using the BB7.7 monoclonal antibody (mAb) coupled to immunomagnetic beads. Molecules were analysed biochemically on SDS-PAGE gels and finally by ID-isoelectric-focusing (IEF). Serum- and sweat-HLA IEF-band patterns of the same individual were fully identical, showing that HLA excreted in sweat possess polymorphic structures like those in serum. Quantitatively, we used a highly sensitive competitive enzyme-linked immunosorbent (ELISA) assay to determine soluble class I concentrations. The first group was that of non-HLA-A9 and -Bw62 sera, which were found to contain HLA levels with a mean concentration of 0.82 +/- 0.63 microgram/ml (n = 44). However, sera that were HLA-A23 or -24 (splits of HLA-A9) contained higher levels, with a mean of 3.2 +/- 0.94 microgram/ml (n = 20). Similarly, HLA-Bw62 individuals had a higher mean of 2.05 +/- 0.65 micrograms/ml (n = 10). The difference of the HLA-A9 group to the first group was statistically highly significant, P less than 0.0001, and that of the HLA-Bw62 to the first was also significant, P less than 0.004. Individuals who were both HLA-A9 and -Bw62 (n = 5) did not express significantly higher levels than those...Continue Reading

References

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Citations

Jul 1, 1994·Human Immunology·M HagiharaK Tsuji
Jun 1, 1997·Physiology & Behavior·B M PauseR Ferstl
Jul 31, 1998·Human Immunology·J C McDonald, I Adamashvili
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Jun 24, 2006·American Journal of Reproductive Immunology : AJRI·Amy LindamanNicholas Zavazava
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Oct 8, 2004·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Charles J Wysocki, George Preti
Jan 1, 1994·Journal of Clinical Laboratory Analysis·N ZavazavaW Müller-Ruchholtz
Mar 25, 2014·Infancy : the Official Journal of the International Society on Infant Studies·Rachel L HuttElizabeth J Kiel
Jun 17, 2006·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Minal A Patel, Surendra S Katyare
Jan 1, 1993·Clinical and Experimental Immunology·R HausmannW Müller-Ruchholtz

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