PMID: 8652Aug 1, 1976

Characterization of some pneumococcal bacteriophages

Journal of Virology
R D Porter, W R Guild

Abstract

The growth of pneumococcal phages at high cell and phage densities is enhanced strongly by the substitution of potassium for sodium in the medium. Initial titers of 2 X 10(10) to 4 X 10(10) PFU/ml are readily obtained, and concentrated stocks are stable in a storage buffer described here. The mechanism of the cation effect is obscure. Phages omega3 and omega8 each have linear double-stranded DNA of 33 X 10(6) daltons per particle, with an apparent guanine plus cytosine content of 47 to 49 mol%, as determined by buoyancy and melting temperature, but with an unusual absorbance spectrum. Efficiency of plating is high if sufficient time is allowed for a relatively slow adsorption, which differs several-fold in rate between the two phages. Morphologically, these and other pneumococcal phages are similar to coliphage lambda but with a longer tail and tail fiber. Upon UV inactivation, omega3 and omega8 have D37 values of 33 and 55 J/m2, respectively, and each shows multiplicity reactivation. A total of 13 ts mutants have been isolated from the two phages, representing only two complementation groups; complementation and recombination occur between omega3 and omega8 mutants. Both phages provoke high-titer antisera with extensive cross-...Continue Reading

Citations

Jan 15, 1996·FEMS Microbiology Letters·R ProvvediG Pozzi
Jun 15, 2000·Journal of Bacteriology·M M Samrakandi, F Pasta
Jun 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Sébastien GuiralJean-Pierre Claverys
Mar 3, 2010·Infection and Immunity·Merit MelinMerja Väkeväinen
Jul 19, 2002·Molecular Microbiology·Mathieu BergéJean-Pierre Claverys
Jul 17, 2004·Molecular Microbiology·Lauren J McAllisterJames C Paton
Jul 25, 2006·Infection and Immunity·Jutta M Loeffler, Vincent A Fischetti
Jan 1, 1994·Journal of Basic Microbiology·R S KingA T Phillips
Sep 27, 2012·The Journal of Biological Chemistry·Marco CassoneMichael E Sebert

Related Concepts

Adsorption
Bacteriophages
Cytosine
DNA, Viral
Cis-Trans Test
Guanine
Potassium
Recombination, Genetic
Sodium
Streptococcus pneumoniae

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