Characterization of Spanish Trichinella isolates by random amplified polymorphic DNA (RAPD)

Journal of Helminthology
E RodríguezT Garate

Abstract

The random amplified polymorphic DNA (RAPD) assay was used to find molecular markers able to distinguish Trichinella spiralis from T. britovi, the two recognized Spanish Trichinella species. Fourteen Spanish Trichinella isolates, as well as reference Trichinella isolates representing the five species T. spiralis (T1), T. nativa (T2), T. britovi (T3), T. pseudospiralis (T4) and T. nelsoni (T7) and the three other taxa Trichinella T5, Trichinella T6 and Trichinella T8 of the genus, were characterized by RAPD using both purified and crude DNAs from infective muscle larvae (ML) and seven arbitrary primers. Three primers yielded diagnostic RAPD markers for the Spanish T. spiralis and T. britovi isolates as well as for the Trichinella reference isolates analysed, and in the case of crude DNAs the results were obtained in few hours. In addition, the species-specificity of the diagnostic RAPD markers from Spanish Trichinella isolates was studied by cross-hybridization assays. These assays confirmed that the selected diagnostic DNA fragments were not species-specific, but showed potential differences in the copy number among the examined Trichinella genetic clusters.

References

Jan 1, 1991·Parasitology Research·T GarateR M Parkhouse
Nov 25, 1990·Nucleic Acids Research·J G WilliamsS V Tingey
Dec 25, 1990·Nucleic Acids Research·J Welsh, M McClelland
Jun 1, 1990·Trends in Genetics : TIG·D M HeeryR Powell
Aug 1, 1982·Molecular and Biochemical Parasitology·A J SimpsonT F McCutchan
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Dec 15, 1994·Nature·L Iversen
Jan 1, 1994·Molecular and Biochemical Parasitology·P J TigheD I Pritchard
Jan 1, 1993·Parasitology Research·C SouléE Pozio
Jan 1, 1993·Molecular and Biochemical Parasitology·E Dias NetoA J Simpson
Feb 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M TibayrencF J Ayala
Apr 1, 1993·The American Journal of Tropical Medicine and Hygiene·E PozioF Bruschi
Oct 1, 1995·International Journal for Parasitology·E PozioC M Kapel
Dec 1, 1995·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·E RodriguezT Garate
Dec 1, 1993·Parasitology Today·J Dupouy-CametC Soulé

❮ Previous
Next ❯

Citations

Dec 2, 2000·Veterinary Parasitology·D S Zarlenga, G La Rosa
Jan 20, 2010·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Kristen M Webb, Benjamin M Rosenthal
Jun 20, 1998·Molecular and Cellular Probes·R B GasserE Pozio
Apr 17, 2001·The Korean Journal of Parasitology·H S KwonK H Joo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.