Characterization of specific binding sites for corticosterone in mouse liver plasma membrane

Membrane Biochemistry
M TruebaJ M Macarulla

Abstract

The specific binding of [3H]corticosterone to mouse liver purified plasma membrane fractions is a saturable, reversible, and temperature-dependent process. Only one type of independent and equivalent binding sites has been determined in plasma membrane (Kd = 4.1 nM and Bmax = 3368 fmol/mg). As can be deduced from displacement data obtained in plasma membrane, the high-affinity binding site is different from nuclear glucocorticoid, nuclear progesterone, and Na+, K(+)-ATPase digitalis receptors. Probably this corticosterone binding site or receptor is the same one determined previously for [3H]cortisol in mouse liver plasma membrane. Such beta- and alpha-adrenergic antagonists as propranolol and phentolamine did not affect [3H]corticosterone binding to plasma membranes; therefore, this binding site is independent of these receptors. The binding sites in plasma membranes are not exclusive for corticosterone, but other steroids are also bound with very different affinities.

References

May 1, 1977·The Journal of Endocrinology·B KochC Mialhe
May 1, 1989·The Journal of Membrane Biology·M TruebaJ M Macarulla
Dec 1, 1988·Experimental and Clinical Endocrinology·M J SanchoA Marino
Jul 1, 1986·Experimental and Clinical Endocrinology·A VallejoJ M Macarulla
Dec 1, 1986·Experimental and Clinical Endocrinology·M J SanchoJ M Macarulla
Jan 1, 1987·The International Journal of Biochemistry·A Sanchez-BuenoJ M Macarulla
Jan 1, 1987·The International Journal of Biochemistry·M TruebaJ M Macarulla
Jan 1, 1986·Advances in Experimental Medicine and Biology·A Allera, G S Rao
Feb 28, 1985·Biochimica Et Biophysica Acta·M Savart, Y Cabillic
Feb 26, 1986·Biochemical and Biophysical Research Communications·C J Lovell-Smith, P Garcia-Webb
Nov 1, 1985·Japanese Journal of Pharmacology·H Kaya, T Saito
Jan 1, 1974·Methods in Enzymology·N N Aronson, O Touster
Sep 1, 1984·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·A DiezJ M Macarulla
Jan 1, 1984·International Review of Cytology·C M Szego, R J Pietras
Nov 1, 1981·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·M EgañaJ M Macarulla
Jan 1, 1984·Journal of Steroid Biochemistry·N C LanJ D Baxter
Feb 23, 1984·Nature·W V WelshonsJ Gorski
Mar 1, 1984·Nature·W T Schrader
Feb 1, 1981·Molecular and Cellular Endocrinology·G S Rao
Mar 10, 1980·FEBS Letters·G H JonkersJ C Birkenhäger

❮ Previous
Next ❯

Citations

Dec 1, 1991·Journal of Bioenergetics and Biomembranes·I IbarrolaM Trueba
Mar 1, 1991·The Journal of Membrane Biology·M TruebaJ M Macarulla
Oct 2, 1996·Biochimica Et Biophysica Acta·I IbarrolaM Trueba
Jun 16, 2001·Endocrine Reviews·S F PalterE Y Adashi
Jul 5, 2003·Physiological Reviews·Ralf M LoselMartin Wehling

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.