PMID: 9006656Jan 1, 1997Paper

Characterization of spherical amyloid protein from a prolactin-producing pituitary adenoma

Acta Neuropathologica
D R HintonJ A Garner

Abstract

Prolactin (PRL)-producing pituitary adenomas are in some cases associated with deposition of abundant spherical amyloid; however, the origin of the amyloid has not been established. In this report, a PRL-producing pituitary adenoma composed almost entirely of spherical amyloid was analyzed biochemically. The tumor was removed surgically from a 56-year-old man. Immunohistochemical analysis revealed that residual tumor cells were strongly positive for PRL, while the spherical amyloid was not. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed a band of approximately 4 kDa associated with the amyloid, which was not present in a nonamyloid producing prolactinoma. The 4-kDa band is similar in size to other known amyloidogenic peptides. Immunoblot analysis of the tumor material using polyclonal anti-human PRL antibodies revealed a small amount of normal-sized PRL; however, the abundant 4-kDa band was nonimmunoreactive. Amino acid sequencing showed that this peptide represents the first 34 amino acids of the intact PRL protein with a predicted size of 4313 Da. The presence of a small amount of normal-sized PRL in this tumor, as well as elevated circulating levels of PRL implies that intact PRL is being abnormally proce...Continue Reading

Citations

Jul 3, 2013·Endocrine Pathology·Cristiane M IdaKalman Kovacs
May 24, 2008·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Sanser GulBektas Acikgoz
Feb 28, 2007·BMC Bioinformatics·Oscar Conchillo-SoléSalvador Ventura
Dec 10, 2008·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·Salvatore Benvenga, Fabrizio Guarneri
Jun 8, 2000·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·G T Westermark, P Westermark
Apr 22, 1999·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·P WestermarkD Selkoe
Feb 19, 2008·Clinical Neurology and Neurosurgery·Chang Ohk SungDa Keun Lee
May 13, 2017·Annual Review of Biochemistry·Fabrizio Chiti, Christopher M Dobson
Sep 4, 2003·Journal of Endocrinological Investigation·P WiesliR L Bernays
May 8, 2004·Journal of Endocrinological Investigation·S BenvengaF Trimarchi

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