Mar 30, 2020

Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV

Nature Communications
Xiuyuan OuZhaohui Qian

Abstract

Since 2002, beta coronaviruses (CoV) have caused three zoonotic outbreaks, SARS-CoV in 2002-2003, MERS-CoV in 2012, and the newly emerged SARS-CoV-2 in late 2019. However, little is currently known about the biology of SARS-CoV-2. Here, using SARS-CoV-2 S protein pseudovirus system, we confirm that human angiotensin converting enzyme 2 (hACE2) is the receptor for SARS-CoV-2, find that SARS-CoV-2 enters 293/hACE2 cells mainly through endocytosis, that PIKfyve, TPC2, and cathepsin L are critical for entry, and that SARS-CoV-2 S protein is less stable than SARS-CoV S. Polyclonal anti-SARS S1 antibodies T62 inhibit entry of SARS-CoV S but not SARS-CoV-2 S pseudovirions. Further studies using recovered SARS and COVID-19 patients' sera show limited cross-neutralization, suggesting that recovery from one infection might not protect against the other. Our results present potential targets for development of drugs and vaccines for SARS-CoV-2.

  • References
  • Citations49

References

  • We're still populating references for this paper, please check back later.
  • References
  • Citations49

Citations

Mentioned in this Paper

N protein, Severe acute respiratory syndrome virus
CTSL
Middle East Respiratory Syndrome (Mers)
Receptor
Serum
Pharmacologic Substance
Cross Reactivity Immunity
Coronaviridae
Virus Neutralization [MoA]
Glycoproteins

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Related Papers

Le infezioni in medicina : rivista periodica di eziologia, epidemiologia, diagnostica, clinica e terapia delle patologie infettive
Ali A RabaanAlfonso J Rodriguez-Morales
American Journal of Clinical Pathology
Ahmed BabikerJeannette Guarner
The New England Journal of Medicine
Xiaoxia LuChinese Pediatric Novel Coronavirus Study Team
© 2020 Meta ULC. All rights reserved