PMID: 8587650Aug 1, 1995Paper

Characterization of squid enolase mRNA: sequence analysis, tissue distribution, and axonal localization

Neurochemical Research
J T ChunB B Kaplan

Abstract

Enolase is a glycolytic enzyme whose amino acid sequence is highly conserved across a wide range of animal species. In mammals, enolase is known to be a dimeric protein composed of distinct but closely related subunits: alpha (non-neuronal), beta (muscle-specific), and gamma (neuron-specific). However, little information is available on the primary sequence of enolase in invertebrates. Here we report the isolation of two overlapping cDNA clones and the putative primary structure of the enzyme from the squid (Loligo pealii) nervous system. The composite sequence of those cDNA clones is 1575 bp and contains the entire coding region (1302 bp), as well as 66 and 207 bp of 5' and 3' untranslated sequence, respectively. Cross-species comparison of enolase primary structure reveals that squid enolase shares over 70% sequence identity to vertebrate forms of the enzyme. The greatest degree of sequence similarity was manifest to the alpha isoform of the human homologue. Results of Northern analysis revealed a single 1.6 kb mRNA species, the relative abundance of which differs approximately 10-fold between various tissues. Interestingly, evidence derived from in situ hybridization and polymerase chain reaction experiments indicate that th...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Oct 1, 1979·The Biochemical Journal·B B KaplanA E Gioio
Jul 15, 1992·Biochemical and Biophysical Research Communications·C Sato, G Matsumoto
Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J WayJ Battey
Jan 1, 1991·Methods in Cell Biology·L M Angerer, R C Angerer
Jan 1, 1991·Journal of Neuroscience Research·A GiudittaB B Kaplan
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·G F JirikowskiF E Bloom
Apr 11, 1990·Nucleic Acids Research·L CalìA Giallongo
Jul 5, 1990·European Journal of Biochemistry·A GiallongoS Feo
Dec 1, 1988·The Journal of Cell Biology·G J WistowJ Piatigorsky
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D J LipmanJ D Kececioglu
Sep 1, 1987·Journal of Neurochemistry·C P CapanoB B Kaplan
Apr 1, 1988·Journal of Neuroscience Research·E Van ObberghenR A Lazzarini
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A GiallongoL C Showe
Dec 31, 1985·Biochemical and Biophysical Research Communications·M TanakaK Nakashima
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Jul 1, 1994·Journal of Neurochemistry·A E GioioB B Kaplan
Sep 1, 1993·Journal of Neurochemistry·M CrispinoA Giuditta
Jan 1, 1992·The European Journal of Neuroscience·Evita Mohr, Dietmar Richter
Jan 1, 1986·Neurochemistry International·A GiudittaL Santella

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Citations

Sep 26, 2000·Journal of Neuroscience Research·J R Sotelo-SilveiraJ R Sotelo
Jun 8, 2001·Journal of Neuroscience Research·A E GioioB B Kaplan
Jul 20, 2002·Trends in Neurosciences·Antonio GiudittaEdward Koenig
Mar 2, 1999·Neuroscience·E Koenig, A Giuditta
Jul 9, 2004·Annals of the New York Academy of Sciences·Barry B KaplanAnthony E Gioio
Aug 21, 2013·Developmental Neurobiology·Laura F GumyCasper C Hoogenraad
Jun 13, 2009·Experimental Neurology·Laura F GumyJames W Fawcett
Apr 9, 2008·Physiological Reviews·Antonio GiudittaMarianna Crispino
Apr 13, 2021·Progress in Neurobiology·Carla Perrone-CapanoMarianna Crispino

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