PMID: 7541563Jan 1, 1995Paper

Characterization of stem cells in human airway capable of reconstituting a fully differentiated bronchial epithelium

Somatic Cell and Molecular Genetics
M L ZepedaJ M Wilson

Abstract

The epithelia of the lung are complex structures that play an important role in normal lung physiology and are often involved in the pathophysiology of pulmonary diseases. The dynamics of cell turnover, lineage, and differentiation within these epithelia are complex and poorly understood. We have coupled the technique of retrovirus-mediated gene transfer with a xenograft model of proximal human airway to evaluate pathways of cellular proliferation and differentiation in human bronchial epithelia. Primary isolates of human bronchial epithelial cells (HBECs) were infected with mixtures of recombinant retroviruses expressing different reporter genes and seeded into denuded rat trachea, which were implanted subcutaneously into athymic mice. The HBECs were allowed to regenerate for four weeks in xenografts, which were then explanted. Clonal expansion of individual retrovirus-marked cells in the regenerated human bronchial epithelium was detected as clusters of transgene-expressing cells. Clone size varied with seeding density, resulting in the largest clones comprising 10(3)-10(4) cells. A substantial number of clones showed transgene expression in basal as well as differentiated columnar cells, a finding that appeared independent o...Continue Reading

References

Dec 1, 1992·Seminars in Cell Biology·G J Graham, I B Pragnell
Dec 1, 1992·Seminars in Cell Biology·E K Parkinson
Dec 1, 1992·Seminars in Cell Biology·J A Jankowski, N A Wright
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·N FerryJ M Heard
Dec 1, 1990·American Journal of Respiratory Cell and Molecular Biology·N F Johnson, A F Hubbs
Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J F EngelhardtJ M Wilson
May 1, 1990·The Journal of Experimental Medicine·G Keller, R Snodgrass
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J PriceC Cepko
Nov 1, 1985·The Anatomical Record·E M McDowellB Coleman
Apr 1, 1974·Proceedings of the Society for Experimental Biology and Medicine·B P Lane, R Gordon
Jan 1, 1981·International Review of Cytology·C S Potten
Jan 1, 1983·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·K P KeenanE M McDowell
Mar 1, 1982·Cell and Tissue Kinetics·G M DonnellyC S Heird
Feb 1, 1994·The Journal of Clinical Investigation·J F EngelhardtJ M Wilson
Jul 1, 1994·American Journal of Respiratory Cell and Molecular Biology·T ShimizuY Sakakura

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Citations

Sep 7, 2007·Cell and Tissue Research·Darrell N Kotton, Alan Fine
Oct 23, 2001·Respiratory Research·E Puchelle, B Peault
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·M D DabevaD A Shafritz
Jan 22, 2008·Pulmonary Pharmacology & Therapeutics·Daniel J Weiss
May 1, 2007·Clinics in Chest Medicine·Viranuj SueblinvongDaniel J Weiss
Jul 27, 2005·Stem Cells·Aurélie Avril-DelplanqueBruno Péault
Aug 30, 2008·British Journal of Pharmacology·A VaranouS L Minger
Jul 26, 2008·Stem Cells·Tillie-Louise HackettDarryl A Knight
Apr 23, 2014·Revue des maladies respiratoires·D AdamC Coraux
Nov 25, 2003·Eye·R M Lavker, T-T Sun
Aug 19, 2008·Biomedical Materials·G Turon TeixidorM Madou
Jan 25, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·F DupuitE Puchelle

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