Characterization of structural and functional role of selenocysteine in selenoprotein H and its impact on DNA binding.

Amino Acids
Sagar H BarageVijay B Baladhye

Abstract

Selenoproteins are a group of proteins which contain selenocysteine (Sec or U) in their primary structure. Selenoproteins play a critical role in antioxidant defense, hormone metabolism, immune responses and muscle development. The selenoprotein H (SELENOH) is essential in the regulation of gene expression in response to redox status and antioxidant defense. It has Sec residue located in conserved CXXU motif similar to other selenoproteins. However, exact biological function of Sec residue in SELENOH is not known in detail. Therefore, it is essential to understand the structural and functional role of Sec in SELENOH. In the present study, homology modelling and MD simulation were performed to understand the role of Sec residue in SELENOH. The modelled 3D structure of wild-SELENOH along with two mutants (Mut-U44C and Mut-41CS-SC44) was subjected to MD simulation. Based on simulation results, we demonstrate that wild-SELENOH structure is dynamically stabilized by network of intramolecular hydrogen bonding and internal residue contacts facilitated by Sec residue. In contrast, notable differences have been observed in residue contacts and stability in other two mutant structures. Additionally, docking studies revealed that 3PRGRKRK...Continue Reading

References

Jul 2, 1992·Nature·D T JonesJ M Thornton
Jun 1, 1983·European Journal of Biochemistry·O EppA Wendel
May 1, 1996·Protein Science : a Publication of the Protein Society·D Fischer, D Eisenberg
Feb 1, 1996·Journal of Molecular Graphics·W HumphreyK Schulten
Jan 1, 1996·Annual Review of Biochemistry·T C Stadtman
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Oct 27, 1998·Computers & Chemistry·M J SchoonmanP D Grootenhuis
Jun 7, 2000·Proceedings of the National Academy of Sciences of the United States of America·L ZhaoE W Taylor
Jun 27, 2000·Bioinformatics·L J McGuffinD T Jones
Jun 5, 2001·The Journal of Biological Chemistry·F J Martin-RomeroD L Hatfield
Aug 22, 2002·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·M VendruscoloM Karplus
May 31, 2003·Science·Gregory V KryukovVadim N Gladyshev
Jun 3, 2004·Genome Research·Gavin E CrooksSteven E Brenner
Jun 25, 2004·Nucleic Acids Research·Burkhard RostJinfeng Liu
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Nov 17, 2004·Journal of Molecular Biology·Gil AmitaiShmuel Pietrokovski
Nov 18, 2004·Biochimica Et Biophysica Acta·Hitoshi Nakamoto, James C A Bardwell
Nov 24, 2004·Computational Biology and Chemistry·V A Simossis, J Heringa
Dec 9, 2004·Bioinformatics·Gianluca Pollastri, Aoife McLysaght
Sep 10, 2005·Biophysical Journal·K V Brinda, Saraswathi Vishveshwara
Dec 2, 2005·The Journal of Biological Chemistry·Andrew D FergusonJohann Deisenhofer
Jan 13, 2006·American Journal of Physiology. Cell Physiology·Yoshihiro TakamuraDhirendra P Singh
Nov 1, 2006·Protein Science : a Publication of the Protein Society·Min-Yi Shen, Andrej Sali
Mar 6, 2007·The Journal of Biological Chemistry·Sergey V NovoselovVadim N Gladyshev
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Jul 12, 2007·Bioinformatics·John H MorrisThomas E Ferrin
Sep 2, 2008·The Journal of Biological Chemistry·Jun Lu, Arne Holmgren
Apr 29, 2009·Journal of Computational Chemistry·Garrett M MorrisArthur J Olson
Jul 25, 2009·The Biochemical Journal·Frederick P BellingerMarla J Berry
Sep 15, 2009·Antioxidants & Redox Signaling·Valentina A ShchedrinaVadim N Gladyshev
Jun 8, 2010·Molecular & Cellular Proteomics : MCP·John H MorrisThomas E Ferrin
Oct 12, 2010·Journal of Biotechnology·Joris BeldDonald Hilvert
Apr 2, 2011·Molecular & Cellular Proteomics : MCP·Svetlana DokudovskayaCatherine Dargemont
Oct 29, 2014·Nucleic Acids Research·UNKNOWN UniProt Consortium
May 8, 2015·Nature Protocols·Lawrence A KelleyMichael J E Sternberg
Nov 29, 2015·IUBMB Life·Xiong ZhangWen-Hsing Cheng

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Methods Mentioned

BETA
protein folding
immunoprecipitation

Software Mentioned

PHYRE
SYMPRED
Cytoscape
gromos
YASPIN
RIN analyser
SAVESv4
JNET
Verify3D
AutoDock Tools

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