PMID: 15225140Jul 1, 2004Paper

Characterization of structure and expression of the growth hormone receptor gene of the Japanese flounder (Paralichtys olivaceus)

The Journal of Endocrinology
Nobuhiro NakaoMinoru Tanaka

Abstract

Growth hormone receptor (GHR) cDNA and gene of the Japanese flounder (Paralicthys olivaceus) were cloned and their molecular structures were characterized. The 641 amino acid sequence predicted from the cDNA sequence showed more than 75% overall sequence similarity with GHRs of other teleosts such as turbot and goldfish, and contained common structural features of vertebrate GHRs. The extracellular domain of flounder GHR had three pairs of cysteines and an FGEFS motif with a replacement E to D. The cytoplasmic domain contained two conserved motifs referred to as box 1 and box 2. The flounder GHR gene was cloned by PCR using primers designed from the sequence of the GHR cDNA. The GHR gene was composed of 10 exons. The sequence of exon 1 corresponded to the 5'-untranslated region of the cDNA, and exons 2-6 encoded most parts of the extracellular domain. The transmembrane domain was found in exon 7, and the intracellular domain was encoded in exons 8-10. Exon 10 also encoded the 3'-untranslated region. Comparison of the flounder GHR gene with the human GHR gene shows that the flounder gene contains no exons corresponding to exon 3 of the human GHR gene, and that the region corresponding to exon 10 in the human GHR gene is encoded ...Continue Reading

Citations

Apr 10, 2012·General and Comparative Endocrinology·David A Irwin, Glen Van Der Kraak
Mar 17, 2010·Gene Expression Patterns : GEP·Cecilia M Di PrinzioSilvia E Arranz
Mar 4, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Eduardo FuentesAlfredo Molina
Dec 5, 2006·General and Comparative Endocrinology·Jon HildahlBjörn Thrandur Björnsson
Feb 7, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Yuichi OzakiKohei Yamauchi
Jan 13, 2006·General and Comparative Endocrinology·Tatsuya Sakamoto, Stephen D McCormick

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