Characterization of Su48, a centrosome protein essential for cell division.

Proceedings of the National Academy of Sciences of the United States of America
Qiang WangMark I Greene

Abstract

The centrosome functions as the major microtubule-organizing center and plays a vital role in guiding chromosome segregation during mitosis. Centrosome abnormalities are frequently seen in a variety of cancers, suggesting that dysfunction of this organelle may contribute to malignant transformation. In our efforts to identify the protein components of the centrosome and to understand the structure features involved in the assembly and functions of this organelle, we cloned and characterized a centrosome-associated protein called Su48. We found that a coiled coil-containing subdomain of Su48 was both sufficient and required for its centrosome localization. In addition, this structure also modulates Su48 dimerization. Moreover, ectopic expression of Su48 causes abnormal mitosis, and a mutant form of Su48 disrupts the localization of gamma-tubulin to the centrosome. Finally, by microinjection of an anti-Su48 antibody, we found that disruption of normal Su48 functions leads to mitotic failure, possibly due to centrosome defects or incomplete cytokinesis. Thus, Su48 represents a previously unrecognized centrosome protein that is essential for cell division. We speculate that Su48 abnormalities may cause aberrant chromosome segregati...Continue Reading

References

Aug 24, 1995·Nature
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·W L LingleJ L Salisbury
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·B J SchnackenbergR E Palazzo
Feb 27, 1999·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·T NagaseO Ohara
Oct 16, 1999·Biology of the Cell·J L SalisburyS L Barrett
Nov 24, 1999·The Journal of Cell Biology·F FavaM Wright
Dec 14, 1999·The American Journal of Pathology·W L Lingle, J L Salisbury
Jan 3, 2001·The Journal of Cell Biology·R N GunawardaneY Zheng
Feb 27, 2001·Science·M PielM Bornens
Sep 5, 2001·Nature Reviews. Molecular Cell Biology·S Doxsey
Nov 6, 2001·Molecular Biology of the Cell·S M MurphyT Stearns
Mar 29, 2002·Cell Motility and the Cytoskeleton·Young Y Ou, Jerome B Rattner
May 17, 2002·The Journal of Biological Chemistry·Brent T SteadmanJames R Goldenring
Nov 5, 2002·Nature Reviews. Cancer·Erich A Nigg
May 18, 2004·Molecular Biology of the Cell·Wendy C ZimmermanStephen J Doxsey
Aug 17, 2004·DNA and Cell Biology·Qiang WangMark I Greene

❮ Previous
Next ❯

Citations

Jul 29, 2011·Expert Reviews in Molecular Medicine·Benjamin Pickard
Jan 5, 2011·Neuropharmacology·Nicholas J Bradshaw, David J Porteous
May 5, 2007·The Journal of Biological Chemistry·Norikazu YabutaHiroshi Nojima
Apr 21, 2009·Cell Biology International·Yanrui WuMing Fan
Jun 5, 2019·Scientific Reports·Cillian R KingRuud F Toonen
Jun 19, 2013·Cancer Discovery·Yuqing ZhangJi-Hye Paik

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.