PMID: 7536933Apr 11, 1995Paper

Characterization of subtype-specific antibodies to the human D5 dopamine receptor: studies in primate brain and transfected mammalian cells

Proceedings of the National Academy of Sciences of the United States of America
C BergsonR Levenson

Abstract

To achieve a better understanding of how D5 dopamine receptors mediate the actions of dopamine in brain, we have developed antibodies specific for the D5 receptor. D5 antibodies reacted with recombinant baculovirus-infected Sf9 cells expressing the D5 receptor but not with the D1 receptor or a variety of other catecholaminergic and muscarinic receptors. Epitope-tagged D5 receptors expressed in mammalian cells were reactive with both D5 antibodies and an epitope-specific probe. A mixture of N-linked glycosylated polypeptides and higher molecular-mass species was detected on immunoblots of membrane fractions of D5-transfected cells and also of primate brain. D5 receptor antibodies intensely labeled pyramidal neurons in the prefrontal cortex, whereas spiny medium-sized neurons and aspiny large interneurons of the caudate nucleus were relatively lightly labeled. Antibodies to the D5 dopamine receptor should prove important in experimentally determining specific roles for the D5 and D1 receptors in cortical processes and diseases.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M Gossen, H Bujard
Oct 1, 1992·Brain Research. Molecular Brain Research·G W HuntleyS C Sealfon
Feb 22, 1991·Science·T Sawaguchi, P S Goldman-Rakic
Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D K GrandyO Civelli
Jan 1, 1990·Cold Spring Harbor Symposia on Quantitative Biology·P S Goldman-RakicC J Bruce
Jul 1, 1984·Cell·I A WilsonR A Lerner
Dec 3, 1984·Life Sciences·J C Stoof, J W Kebabian
Dec 21, 1993·Biochemistry·V A Canfield, R Levenson
Mar 15, 1994·European Journal of Pharmacology·G Y NgB F O'Dowd
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·J F SmileyP S Goldman-Rakic
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A I LeveyB J Ciliax

❮ Previous
Next ❯

Citations

Jan 1, 1996·Drug Development Research·A Michiel van Rhee, Kenneth A Jacobson
Mar 25, 2011·Psychopharmacology·Matthew A AlbrechtFlavie Waters
Mar 1, 1996·Neuroscience Letters·T V BeischlagH B Niznik
Feb 18, 1998·Neuroscience·A SidhuY Shin
Sep 28, 1998·The European Journal of Neuroscience·C MaugerF Monsma
Dec 11, 2002·The European Journal of Neuroscience·Alicia RiveraRosario Moratalla
Jun 16, 2010·Pharmacology & Therapeutics·Ashiwel S Undieh
Sep 24, 2004·Progress in Neurobiology·Jeremy K Seamans, Charles R Yang
Jan 31, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Veronica BisagnoFrancisco J Urbano
Jan 15, 2011·Behavioural Brain Research·Zafar U Khan, E Chris Muly
Apr 12, 2005·Biological Psychiatry·Stacy A CastnerPatricia S Goldman-Rakic
Dec 6, 2002·Journal of Neurophysiology·Patrick W Awenowicz, Linda L Porter
Oct 24, 2014·American Journal of Physiology. Renal Physiology·Riley Charles EnnisVan Anthony M Villar
Feb 28, 2018·Frontiers in Neural Circuits·Adrienne MuellerTirin Moore
Feb 11, 1998·Physiological Reviews·C MissaleM G Caron

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.