PMID: 3763439Jul 1, 1986Paper

Characterization of sulfated forms of the pro-opiomelanocortin amino-terminal glycopeptide in rat intermediate lobe cells

Peptides
Y BourbonnaisP Crine

Abstract

Explants of rat neurointermediate lobes were incubated in the presence of radioactive amino acids, sugars or sulfate and the labeled proteins were separated by two-dimensional gel electrophoresis. A double series of acidic peptides (Mr = 16,000-21,500) were identified as variant forms of the amino-terminal glycopeptide of pro-opiomelanocortin (N-POMC). The series of peptides with the higher molecular weights (Mr = 18,000-21,500) contain a tryptic fragment (tentatively identified as the tryptic peptide of the "joining peptide": sequence 77 to 93 of rat POMC) which is absent from the forms of the lower molecular weight series (Mr = 16,000 to 18,000). Pulse-chase studies further showed that the high molecular weight forms of N-POMC could be post-translationally cleaved albeit slowly into the species of Mr = 16,000-18,000 which constitute, at least in part, the final maturation products of the N-terminal region of the precursor molecule. All the variant forms of the N-POMC glycopeptide could be labeled with [35S]sulfate. Our results strongly suggest that most of the sulfate groups are attached to N-linked oligosaccharide side chains of N-POMC. We therefore propose that one of the final maturation products of the N-terminal portion ...Continue Reading

References

Jul 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R E MainsN Ling
Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J L Roberts, E Herbert
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·P CrineM Chrétien
Aug 1, 1979·The Biochemical Journal·N MianP W Kent
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P CrineM Chrétien
Sep 1, 1979·Canadian Journal of Biochemistry·M ChrétienN G Seidah
Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·N G SeidahM Chrétien
Oct 15, 1979·Analytical Biochemistry·M R GoldsmithS C Bock
Oct 4, 1985·Science·R B Kelly
Jun 1, 1984·Bioscience Reports·P J Lowry
Jul 1, 1983·The Journal of Endocrinology·B G JenksA P van Overbeeke
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·V Y Hook, Y P Loh
Jul 1, 1983·Canadian Journal of Biochemistry and Cell Biology = Revue Canadienne De Biochimie Et Biologie Cellulaire·C LazureM Chrétien
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·E D GreenI Boime
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·T F Parsons, J G Pierce
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G HortinI Boime
Jan 1, 1981·Annual Review of Biochemistry·S C Hubbard, R J Ivatt
May 15, 1981·Biochemical and Biophysical Research Communications·C A BrowneS Solomon

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.