PMID: 2497104May 1, 1989Paper

Characterization of synapsin I fragments produced by cysteine-specific cleavage: a study of their interactions with F-actin

The Journal of Cell Biology
M BählerP Greengard

Abstract

Synapsin I is a neuron-specific phosphoprotein that is concentrated in the presynaptic nerve terminal in association with the cytoplasmic surface of synaptic vesicles. It has been demonstrated to bundle F-actin in a phosphorylation-dependent manner in vitro, a property consistent with its proposed role in linking synaptic vesicles to the cytoskeleton and its involvement in the regulation of neurotransmitter release. Synapsin I is composed of two distinct domains, a COOH terminal, collagenase-sensitive, hydrophilic, and strongly basic tail region, and an NH2 terminal, collagenase-resistant head region relatively rich in hydrophobic amino acids. To elucidate the structural basis for the interactions between synapsin I and F-actin and how it relates to other characteristics of synapsin I, we have performed a structure-function analysis of fragments of synapsin I produced by cysteine-specific cleavage with 2-nitro-5-thiocyanobenzoic acid. The fragments were identified and aligned with the parent molecule using the deduced primary structure of synapsin I and the known phosphorylation sites as markers. We have purified these fragments and examined their interactions with F-actin. Two distinct fragments, a 29-kD NH2-terminal fragment ...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jul 1, 1978·Analytical Biochemistry·P T Matsudaira, D R Burgess
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·P MatsudairaE Atherton
Jan 9, 1986·Nature·A J Baines, V Bennett
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R LlinásP Greengard
Dec 15, 1986·Biochemical Pharmacology·P De Camilli, P Greengard
Mar 1, 1986·The Journal of Cell Biology·J A Wilkins, S Lin
Apr 16, 1987·Nature·M Bähler, P Greengard
Sep 1, 1987·The Journal of Cell Biology·T C Petrucci, J S Morrow
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A J CzernikP Greengard
Jan 1, 1988·Journal of Cellular Biochemistry·T C PetrucciJ S Morrow
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L K KaczmarekP Greengard
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R JahnP Greengard
Sep 16, 1980·Biochemical and Biophysical Research Communications·S MacLean-Fletcher, T D Pollard

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Citations

Oct 23, 1997·Protein Science : a Publication of the Protein Society·C R WangJ Deisenhofer
Jan 1, 1992·Molecular Neurobiology·P Paggi, T C Petrucci
Oct 11, 1991·Brain Research. Molecular Brain Research·D S HowlandL J DeGennaro
Jul 1, 1993·Neurochemistry International·F Benfenati, F Valtorta
Mar 2, 2010·Journal of the American Society for Mass Spectrometry·Peiran LiuReb J Russell
Feb 5, 1998·Brain Research. Molecular Brain Research·P VaccaroF Benfenati
Jun 24, 2000·Biochimie·F Doussau, G J Augustine
Oct 1, 1996·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C L HartleyS M Thomas
Feb 15, 2001·The Biochemical Journal·J J CheethamA J Czernik
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T S SihraP Greengard
Jan 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·F BenfenatiP Greengard
Aug 1, 1993·The Journal of Cell Biology·J M Ervasti, K P Campbell
Apr 23, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·S HilfikerP Greengard
Mar 28, 1998·The European Journal of Neuroscience·H B NielanderF Benfenati
Mar 9, 1994·Annals of the New York Academy of Sciences·F Valtorta, F Benfenati
Oct 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·F OnofriF Benfenati
May 1, 1991·Journal of Neurochemistry·T LüthiM Bähler
May 21, 2003·The Journal of Cell Biology·Ona BloomOleg Shupliakov
Jun 1, 1990·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M BählerP Greengard
Nov 14, 2015·FEBS Open Bio·William L ColemanJennifer J Venditti
Jul 30, 2011·Seminars in Cell & Developmental Biology·Oleg ShupliakovArndt Pechstein
Jul 30, 2011·Seminars in Cell & Developmental Biology·Flavia ValtortaEugenio F Fornasiero
May 5, 2011·Molecular and Cellular Neurosciences·Ona E Bloom, Jennifer R Morgan
Jul 16, 2008·Journal of Molecular Biology·Gustavo SajnaniJesús R Requena

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