Characterization of synthetic lung surfactant activity against proinflammatory cytokines in human monocytes

Biological & Pharmaceutical Bulletin
Eiji OtsuboMasato Nomura

Abstract

Our previous study demonstrated that the smallest synthetic peptide with the sequence CPVHLKRLLLLLLLLLLLLLLLL, SP-CL16(6-28), admixed with phospholipid (synthetic lung surfactant, SLS) showed strong surface activity. In this study, we attempted to develop a dual-type surfactant with both anti inflammatory and surface activities. SP-CL16(6-28) was first chemically synthesized and then purified for use by centrifugal partition chromatography. A mixture of SP-CL16(6-28) and phospholipid complex was tested for anti inflammatory activity using the human monocyte cell line THP-1. Whether the suppression of tumor necrosis factor-alpha (TNF-a), interleukin (IL)-8, IL-6, IL-1beta, and macrophage migration inhibitory factor (MIF) was reduced by lipopolysaccharide (LPS) in monocytes was examined. Levels of these cytokines were measured by enzyme-linked immunosorbent assay. It was found that SLS significantly and dose dependently inhibited the secretion of TNF-alpha by THP-1 cells following stimulation with LPS. Dipalmitoylphosphatidylcoline did not inhibit the release of cytokines. These findings suggest that SLS has anti inflammatory activity. Therefore it should be possible to develop a SLS with both anti inflammatory activity and surfa...Continue Reading

References

Sep 1, 1992·American Journal of Respiratory Cell and Molecular Biology·M J ThomassenH P Wiedemann
Mar 1, 1990·The American Review of Respiratory Disease·J Kelley
Oct 1, 1988·The American Review of Respiratory Disease·F Possmayer
Dec 17, 1987·The New England Journal of Medicine·G R BernardC A Metz
Dec 16, 1974·Biochimica Et Biophysica Acta·J Goerke
Jul 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·J R David
Jan 1, 1984·Experimental Lung Research·A B Fisher, A Chander
Sep 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S A RooneyC R Mendelson
Apr 1, 1994·American Journal of Respiratory Cell and Molecular Biology·M J ThomassenH P Wiedemann
Jun 1, 1993·The American Review of Respiratory Disease·J Tran Van NhieuJ F Bernaudin
Apr 1, 1996·American Journal of Respiratory Cell and Molecular Biology·J M AntalM J Thomassen
Dec 1, 1996·Biological & Pharmaceutical Bulletin·T TakeiH Ohkawa

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Citations

Jul 28, 2020·Pharmaceutics·Ioannis AnestopoulosMihalis I Panayiotidis

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