PMID: 8985324Jan 1, 1997Paper

Characterization of T-cell response to woodchuck hepatitis virus core protein and protection of woodchucks from infection by immunization with peptides containing a T-cell epitope

Journal of Virology
S MenneM Roggendorf

Abstract

Specific activation of T cells appears to be a prerequisite for viral clearance during hepatitis B virus (HBV) infection. The T-cell response to HBV core protein is essential in determining an acute or chronic outcome of HBV infection, but how this immune response contributes to the course of infection remains unclear. This is due to results obtained from humans, which are restricted to phenomenological observations occurring during the clinical onset after HBV infection. Thus, a useful animal model is needed. Characterization of the T-cell response to the core protein (WHcAg) of woodchuck hepatitis virus (WHV) in woodchucks contributes to the understanding of these mechanisms. Therefore, we investigated the response of woodchuck peripheral blood mononuclear cells (PBMCs) to WHcAg and WHcAg-derived peptides, using our 5-bromo-2'-deoxyuridine assay. We demonstrated WHcAg-specific proliferation of PBMCs and nylon wool-nonadherent cells from acutely WHV-infected woodchucks. Using a cross-reacting anti-human T-cell (CD3) antiserum, we identified nonadherent cells as woodchuck T cells. T-cell epitope mapping with overlapping peptides, covering the entire WHcAg, revealed T-cell responses of acutely WHV-infected woodchucks to peptide1...Continue Reading

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Citations

Aug 4, 1999·Antiviral Chemistry & Chemotherapy·R F SchinaziS Dagan
Apr 2, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Yun WangPaul J Cote
May 15, 2007·Liver International : Official Journal of the International Association for the Study of the Liver·Hyung Joon KimByung Chul Yoo
May 1, 2020·Viruses·Jerri C CaldeiraFederica Cavallo
Feb 14, 2002·The Journal of General Virology·Elisenda ArmengolArmin Saalmüller
Jul 26, 2007·Journal of Viral Hepatitis·A SchumannM Lindemann
Jun 15, 2001·ILAR Journal·B C Tennant, J L Gerin
Jan 20, 2016·Viruses·Dvora Witt-KehatiAmir Shlomai
Aug 12, 2010·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Yu ZhuHong Tu

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