Characterization of T cell responses to fragrances

Toxicology and Applied Pharmacology
S SiebenB Blömeke

Abstract

Fragrances are worldwide a major cause of allergic contact dermatitis (ACD), a delayed-type hypersensitivity reaction mediated by T lymphocytes. We investigated T cell responses to fragrances using peripheral blood mononuclear cells (PBMC) and T cells from skin lesions of fragrance-allergic patients. The components of a fragrance mixture (eugenol, isoeugenol, geraniol, oak moss, alpha-amyl cinnamic aldehyde, cinnamic aldehyde, cinnamic alcohol, and hydroxycitronellal) that is commonly used in the patch test were studied in vitro in the lymphocyte transformation test (LTT). PBMC from fragrance-allergic patients (n = 32) showed significant stimulations to all eight fragrances. The calculated stimulation indices (SI) varied between 2.1 and 21.8. The influence of metabolic enzymes on T cell stimulation was studied for two fragrances. Interestingly, stimulation of eugenol and isoeugenol was increased in the presence of antigen-modified human liver microsomes (CYP450) or recombinant CYP1A1 in five of seven cases. Furthermore, we established 18 T cell clones (TCC) from a skin lesion reacting specifically to eugenol. FACS analysis revealed that the majority (n = 15, 83%) of TCC were CD3(+), CD4(+), and HLA-DR(+). Seventeen percent (n =...Continue Reading

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Citations

Mar 20, 2009·Natural Product Research·T G RodriguesJ M Sforcin
Oct 22, 2009·Natural Product Research·Tatiana Fernanda BachiegaJosé Maurício Sforcin
Jul 12, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jenny HennenBrunhilde Blömeke
Jun 13, 2012·Journal of Toxicology and Environmental Health. Part a·Michaela Kalmes, Brunhilde Blömeke
Apr 29, 2014·The Journal of Allergy and Clinical Immunology·Nikhil DhingraEmma Guttman-Yassky
Nov 30, 2004·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·H F Merk, J M Baron
Nov 12, 2005·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·R Brehler, H Merk
Dec 23, 2008·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·R BransB Blömeke
Jul 5, 2011·Immunopharmacology and Immunotoxicology·Yu-Ping CaoZi-Yi Chen
Nov 25, 2006·The Journal of Investigative Dermatology·Moa Andresen BergströmJens M Baron
Oct 11, 2008·Contact Dermatitis·Claudia SkazikBrunhilde Blömeke
Aug 3, 2006·Experimental Dermatology·Hans F MerkJean Krutmann
Apr 20, 2007·Contact Dermatitis·Jonathan M L WhiteJohn P McFadden
Mar 15, 2006·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Sung Su YeaChul-Ho Yun
Apr 5, 2012·Journal of Immunotoxicology·Christine JäckhRobert Landsiedel
Mar 17, 2004·Toxicology and Applied Pharmacology·H F MerkJ Krutmann

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