Characterization of termite lipophorin and its involvement in hydrocarbon transport

Journal of Insect Physiology
Yongliang FanAnne-Geneviève Bagnères

Abstract

The transport of lipids constitutes a vital function in insects and requires the plasma lipoprotein lipophorin. In all insects examined to date, cuticular hydrocarbons are also transported through the hemolymph by lipophorin, and in social insects they play important roles not only in water proofing the cuticle but also in nestmate recognition. High-density lipophorin (HDLp), isolated from Reticulitermes flavipes plasma by KBr gradient ultracentrifugation, contains 66.2% protein and 33.8% lipids; hydrocarbons constitute its major neutral lipid (20.4% of total lipids). Anti-lipophorin serum was generated in rabbit and its specific association with lipophorin, and not with any other plasma proteins, was verified with Western blotting. Immunoprecipitation also confirmed that this antibody specifically recognizes lipophorin, because all hemolymph hydrocarbons of the termites R. flavipes and R. lucifugus and the cockroach Supella longipalpa, which associate only with lipophorin, were recovered in the immunoprecipitated protein. Cross-reactivity of the antiserum with lipophorin from related species was investigated by double immunodiffusion with 10 termite species in the genera Reticulitermes, Coptotermes, Zootermopsis, and Kaloterme...Continue Reading

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Citations

Apr 9, 2013·Journal of Insect Physiology·Ana Maria Costa-LeonardoIves Haifig
Dec 14, 2006·Annual Review of Entomology·J Chad Gore, Coby Schal
Feb 11, 2011·Archives of Insect Biochemistry and Physiology·Rachel SantosKatia C Gondim
Jan 31, 2013·Archives of Insect Biochemistry and Physiology·David MajerowiczKatia C Gondim
Feb 12, 2005·Insect Biochemistry and Molecular Biology·Michael E ScharfGary W Bennett
Nov 18, 2015·BMC Evolutionary Biology·Ricardo Caliari OliveiraTom Wenseleers
Jul 18, 2019·Proceedings. Biological Sciences·Vasileia BalabanidouJohn Vontas
Nov 3, 2020·Journal of Insect Physiology·Jian-Wen QiaoTong-Xian Liu

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