Characterization of the 5'-flanking region of the gene for the cAMP-inducible protein kinase A subunit, RIIbeta, in Sertoli cells

Molecular and Cellular Endocrinology
H K KnutsenK A Taskén

Abstract

Activation of cyclic AMP-dependent protein kinases (protein kinase A, PKA) by gonadotropins and cyclic AMP (cAMP) plays an important role in the regulation of testicular functions. A regulatory subunit, RIIbeta, of PKA is transcriptionally induced in rat Sertoli cells in response to treatment with cAMP. The present study addresses regulatory mechanisms leading to increased transcription of the rat RIIbeta gene. We have localized a footprint which overlaps one of the major transcription initiation sites in the basal promoter (-293 to -123). One of the proteins binding this sequence belongs to the NF-1 family of transcription factors. We also observed binding to a basic helix-loop-helix (bHLH) response element. Furthermore, transfection studies of various 5'-deletions of the rat RIIbeta gene in primary cultures of rat Sertoli cells and in peritubular cells revealed the presence of an upstream region (-723 to -395, cAMP-responsive region) inhibiting basal expression from the rat RIIbeta gene only in Sertoli cells. This region was found to enhance cAMP responsiveness in Sertoli cells but not in peritubular cells. Interactions with downstream elements seemed to be important for the function of the cAMP-responsive region. Although so...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Mar 1, 1992·The International Journal of Biochemistry·G H LuW D Wicks
Jan 1, 1991·Genes & Development·V E AvvedimentoM E Gottesman
Apr 7, 1989·Cell·S T Smale, D Baltimore
Apr 28, 1989·Biochemical and Biophysical Research Communications·C HansenD Christophe
Jun 1, 1989·Molecular and Cellular Endocrinology·D ChristopheG Vassart
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M E JohnM R Waterman
Apr 1, 1973·Virology·F L Graham, A J van der Eb
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·W H WalkerJ F Habener
Jan 1, 1994·Annual Review of Physiology·S H Francis, J D Corbin
Feb 1, 1993·Computer Applications in the Biosciences : CABIOS·D S Prestridge, G Stormo

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Citations

Aug 26, 1998·Annual Review of Nutrition·P B DanielJ F Habener
Jun 28, 2002·BMC Molecular Biology·Maria Krudtaa DahleKristin Austlid Taskén

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