PMID: 15229358Jul 2, 2004Paper

Characterization of the 5'-flanking transcriptional regulatory region of chicken growth hormone gene

Experimental Biology and Medicine
Stephen C Y IpFrederick C Leung

Abstract

A 1727-bp fragment of 5'-flanking region of chicken growth hormone (cGH) gene has been cloned and sequenced. Various lengths of the 5'-flanking region (122 to 1775 bp) was linked to a luciferase reporter gene, and its transcriptional regulation was examined by an in vitro transient transfection coupled with luciferase assay. Our results demonstrated that pituitary-specific transcription factor, Pit-1, is necessary and sufficient to confer a strong tissue-specific expression. Co-transfection with goldfish or chicken Pit-1 expression vectors significantly restored the luciferase expression in HeLa cells. Site-directed mutagenesis and mobility gel-shift assays further confirmed the position of the Pit-1 binding site at -113/-104. Moreover, a repressive thyroid hormone response element (TRE) was identified at -137/-74, and we propose that interactions between the TRE and Pit-1 sites may be required for its repressive effect.

References

Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·H J SteinfelderF E Wondisford
Jan 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·M N TreacyF Martin
Jul 9, 1990·Molecular and Cellular Endocrinology·A MorinJ A Martial
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Jan 1, 1985·Journal of Animal Science·C S ChungJ P Wiggins
Aug 4, 1999·Molecular and Cellular Endocrinology·B SekkaliM Muller
Jun 8, 2001·Experimental Biology and Medicine·S C IpF C Leung

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Citations

Jan 18, 2011·Endocrinology·Monika Proszkowiec-WeglarzTom E Porter
Feb 15, 2020·The Journal of Poultry Science·Sadequllah AhmadiTakeshi Ohkubo
Dec 14, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Kristina Heuck-KnubelTom E Porter

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