Characterization of the 5'-regulatory region of the human thiamin transporter SLC19A3: in vitro and in vivo studies

American Journal of Physiology. Gastrointestinal and Liver Physiology
Svetlana M Nabokina, H M Said

Abstract

Transcriptional regulation of expression of the human thiamin transporter-2 (the product of the SLC19A3 gene) is unknown. In this study, we cloned the 5'-regulatory region of the human SLC19A3 gene (2,016 bp), identified the minimal promoter region required for basal activity, demonstrated a critical role for specific cis-regulatory elements in determining the promoter activity, and confirmed activity and physiological relevance of the cloned SLC19A3 promoter in vivo. With the use of transiently transfected human intestinal epithelial Caco-2 cells and 5'-deletion analysis, the minimal promoter region required for basal activity of the SLC19A3 promoter was found to be encoded in a sequence between -77 and +59 by using the start of transcription initiation as position 1. This minimal region was found to contain a number of putative cis-regulatory elements, with a critical role for a stimulating protein-1 (SP1)/GC-box binding site (at position -48/-45 bp) established by means of mutational analysis. With the use of EMSA and supershift assays, the binding of SP1 and SP3 to the minimal promoter region was also demonstrated. In transiently transfected Drosophila SL2 cells, both SP1 and SP3 transactivated the SLC19A3 minimal promoter ...Continue Reading

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Citations

Mar 22, 2006·Evidence-based Complementary and Alternative Medicine : ECAM·Derrick Lonsdale
Oct 1, 2011·American Journal of Physiology. Cell Physiology·Arundhati BiswasHamid M Said
May 9, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Lisa MeeHamid M Said
May 8, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Sandeep B SubramanyaHamid M Said
Jul 25, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Balasubramaniem AshokkumarHamid M Said
Aug 13, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Svetlana M NabokinaHamid M Said
Aug 27, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Sandeep B SubramanyaHamid M Said
Dec 24, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Veedamali S SubramanianHamid M Said
Apr 28, 2007·The Journal of Physiology·Veedamali S SubramanianHamid M Said
Mar 7, 2013·CNS Neuroscience & Therapeutics·Khanh V Q Luong, Lan T H Nguyễn
Jan 1, 2016·Diabetes·Massimo PortaUNKNOWN FinnDiane Study Group
Jul 14, 2011·The Biochemical Journal·Hamid M Said
Jan 1, 2013·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Khanh vinh quốc L Ng, Lan Thi Hoàng Nguyễn
Jan 28, 2014·Biochemistry·Sergio ManzettiDavid van der Spoel
Jan 6, 2012·American Journal of Alzheimer's Disease and Other Dementias·Khanh vinh quoc Lu'o'ng, Lan Thi Hoang Nguyen
Feb 12, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jack C Reidling, Hamid M Said
Feb 8, 2006·Current Opinion in Gastroenterology·Hamid M Said, Zainab M Mohammed
May 18, 2006·American Journal of Physiology. Renal Physiology·Balasubramaniem AshokkumarHamid M Said
Mar 29, 2019·American Journal of Physiology. Gastrointestinal and Liver Physiology·Padmanabhan SrinivasanHamid M Said
Aug 31, 2013·American Journal of Physiology. Gastrointestinal and Liver Physiology·Svetlana M NabokinaHamid M Said
Oct 18, 2013·American Journal of Physiology. Cell Physiology·Abhisek GhosalHamid M Said
May 21, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jin ShimakuraKen-Ichi Inui
Sep 1, 2015·American Journal of Physiology. Gastrointestinal and Liver Physiology·Padmanabhan SrinivasanHamid M Said
Nov 16, 2019·Diabetes & Vascular Disease Research : Official Journal of the International Society of Diabetes and Vascular Disease·Elena BeltramoMassimo Porta
Feb 15, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Padmanabhan SrinivasanHamid M Said
Nov 5, 2020·American Journal of Physiology. Gastrointestinal and Liver Physiology·Kasin Yadunandam AnandamHamid M Said

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