Characterization of the 820-nm transmission signal paralleling the chlorophyll a fluorescence rise (OJIP) in pea leaves

Functional Plant Biology : FPB
Gert SchanskerReto J Strasser

Abstract

Monitoring transmission changes at 820 nm, a measure of the redox states of plastocyanin (PC) and P700, is a good complementary technique for chlorophyll (chl) a fluorescence induction measurements. A thorough characterization of the properties of the 820-nm transmission kinetics during the first second after a dark-to-light transition is provided here for pea (Pisum sativum L.) leaves. The data indicate that plastocyanin in a dark-adapted leaf is in the reduced state. Three photosystem I (PSI)-related components, PC, P700 and ferredoxin, can contribute to the 820-nm transmission signal. The contribution of ferredoxin, however, is only approximately 5%, thus, it can be neglected for further analysis. Here, we show that by monitoring the sequential oxidation of PC and P700 during a far-red pulse and analysing the re-reduction kinetics it is possible to assign the three re-reduction components to PC (τ = 7-14 s) and P700 (τ = 35-55 ms and 1.2-1.6 s). Our data indicate that the faster re-reduction phase (τ =35-55 ms) may represent a recombination reaction between P700+ and the acceptor side of PSI. This information made it possible to show that the ratio between the potential contributions of PC : P700 is 50 : 50 in pea and Camell...Continue Reading

Citations

Oct 24, 2019·Annals of Botany·Alexandrina StirbetGovindjee Govindjee
Jun 24, 2009·Photosynthesis Research·Vasilij GoltsevReto J Strasser
Feb 13, 2016·Physiologia Plantarum·Walter Zegada-LizarazuAndrea Monti
Feb 3, 2018·Environmental Science and Pollution Research International·Jingjing ChuXinhao Huang
Oct 1, 2017·Functional Plant Biology : FPB·Rupal Singh Tomar, Anjana Jajoo
Jul 20, 2012·Photosynthesis Research·Alexandrina Stirbet, Govindjee
Sep 11, 2013·American Journal of Botany·Jian-Chao YangBao-Shan Wang
Dec 4, 2012·Physiologia Plantarum·Walter Zegada-Lizarazu, Andrea Monti
Apr 27, 2019·Functional Plant Biology : FPB·Rupal S Tomar, Anjana Jajoo
Jun 27, 2020·Scientific Reports·Alonso ZavaferMarina Gavilanes-Ruiz
May 10, 2007·Photosynthesis Research·Szilvia Z TóthReto J Strasser
Apr 19, 2008·Photosynthesis Research·T Antal, A Rubin
Jul 29, 2005·Photosynthesis Research·David JolyRobert Carpentier

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