Characterization of the A2B adenosine receptor from mouse, rabbit, and dog.

The Journal of Pharmacology and Experimental Therapeutics
John A AuchampachGarren E Maas

Abstract

We have cloned and pharmacologically characterized the A(2B) adenosine receptor (AR) from the dog, rabbit, and mouse. The full coding regions of the dog and mouse A(2B)AR were obtained by reverse transcriptase-polymerase chain reaction, and the rabbit A(2B)AR cDNA was obtained by screening a rabbit brain cDNA library. It is noteworthy that an additional clone was isolated by library screening that was identical in sequence to the full-length rabbit A(2B)AR, with the exception of a 27-base pair deletion in the region encoding amino acids 103 to 111 (A(2B)AR(103-111)). This 9 amino acid deletion is located in the second intracellular loop at the only known splice junction of the A(2B)AR and seems to result from the use of an additional 5' donor site found in the rabbit and dog but not in the human, rat, or mouse sequences. [(3)H]3-Isobutyl-8-pyrrolidinoxanthine and 8-[4-[((4-cyano-[2,6-(3)H]-phenyl)carbamoylmethyl)oxy]phenyl]-1,3-di(n-propyl)xanthine ([(3)H]MRS 1754) bound with high affinity to membranes prepared from human embryonic kidney (HEK) 293 cells expressing mouse, rabbit, and dog A(2B)ARs. Competition binding studies performed with a panel of agonist (adenosine and 2-amino-3,5-dicyano-4-phenylpyridine analogs) and antag...Continue Reading

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Citations

Jun 23, 2011·The Journal of Pharmacology and Experimental Therapeutics·Dharini van der HoevenJohn A Auchampach
Oct 10, 2012·Infection and Immunity·Cirle A WarrenRichard L Guerrant
Jul 12, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Enbo ZhanRobert D Lasley
Oct 12, 2010·American Journal of Physiology. Heart and Circulatory Physiology·P Charukeshi ChandrasekeraRobert D Lasley
May 31, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Diane S Allen-GipsonTodd A Wyatt
Dec 28, 2010·Biochimica Et Biophysica Acta·Christa E Müller, Kenneth A Jacobson
Jun 30, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Ilze BotAdriaan P Ijzerman
May 18, 2013·Journal of Leukocyte Biology·Hjalmar R BoumaRobert H Henning
Feb 13, 2014·British Journal of Pharmacology·Stephen P H AlexanderUNKNOWN CGTP Collaborators
Mar 19, 2014·The Journal of Pharmacology and Experimental Therapeutics·Sonja HinzChrista E Müller

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