Characterization of the allergen group VI in timothy grass pollen (Phl p 6). I. Immunological and biochemical studies

International Archives of Allergy and Immunology
A PetersenW M Becker

Abstract

For the identification and characterization of allergen grass group VI we performed two-dimensional immunoblotting of timothy grass pollen (Phleum pratense). Two intense 13-kD protein spots of pI 5.2 and 5.5 were found to be IgE reactive. By N-terminal microsequencing and amino acid analysis we identified them as Phl p 6 isoallergens. An antiserum was raised against Phl p 6 by immunizing BALB/c mice with allergen bearing nitrocellulose particles of one isoform. The antiserum revealed an intense reactivity to Phl p 6 isoforms, but also showed a weak cross-reactivity with Phl p 5 allergens. After immunoabsorption of patients' serum to Phl p 6 spots on the blotting membrane, we were able to demonstrate that the eluted human IgE antibodies cross-react with the grass group V allergens as well. Therefore, Phl p 5 and Phl p 6 possess one or more common IgE binding epitopes.

Citations

Jun 13, 2015·PloS One·Priscila Ferreira de Sousa MoreiraVerena Niederberger
Apr 15, 1999·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Z N ChangS H Han
Jun 17, 2006·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Arnd PetersenWolf-Meinhard Becker
Jan 1, 1998·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals· Elizabeth I Opara Sarah L Oehlschlager A Bryan Hanley
May 20, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·U SeitzerJ Ahmed
Jun 18, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Carla OseroffAlessandro Sette
May 10, 2001·The Journal of Allergy and Clinical Immunology·A PetersenW M Becker
Dec 4, 2004·The Journal of Allergy and Clinical Immunology·Brigitte HantuschErika Jensen-Jarolim

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