Characterization of the amino acid residues of sendai virus C protein that are critically involved in its interferon antagonism and RNA synthesis down-regulation

Journal of Virology
Atsushi KatoYoshiyuki Nagai

Abstract

Sendai virus (SeV) encodes two accessory proteins, V and C, in the alternative reading frames in the P gene that are accessed transcriptionally (V) or translationally (C). The C protein is expressed as a nested set of four C-coterminal proteins, C', C, Y1, and Y2, that use different initiation codons. Using HeLa cell lines constitutively expressing the various C proteins, we previously found that the smallest (the 175-residue Y2) of the four C proteins was fully capable of counteracting the antiviral action of interferons (IFNs) and inhibiting viral RNA synthesis and that the C-terminal half of 106 residues was sufficient for both of these inhibitory functions (A. Kato et al., J. Virol. 75:3802-3810, 2001, and A. Kato et al., J. Virol. 76:7114-7124, 2002). Here, we further generated HeLa cell lines expressing the mutated C (Cm) proteins with charged amino acids substituted for alanine residues at either positions 77 and 80; 114 and 115; 139 and 142; 151, 153, and 154; 156; or 173, 175, and 176. We found that only the mutations at positions 151, 153, and 154 abolished IFN antagonism. All the Cm proteins lost the ability to bind with STAT1 under our assay conditions, regardless of their ability to inhibit IFN signaling. On the ot...Continue Reading

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Citations

Sep 8, 2011·Proceedings of the National Academy of Sciences of the United States of America·Masaharu Iwasaki, Yusuke Yanagi
May 13, 2006·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Mounira K Chelbi-AlixDanielle Blondel
May 28, 2005·Journal of Virology·Achut G MalurAmiya K Banerjee
Mar 16, 2005·Journal of Virology·Toru KubotaAtsushi Kato
Dec 17, 2008·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Takemasa SakaguchiYoshiyuki Nagai
Nov 10, 2015·Molecular Therapy : the Journal of the American Society of Gene Therapy·Li-Wen LiuYasufumi Kaneda
Sep 24, 2013·Journal of Molecular Biology·Griffith D Parks, Martha A Alexander-Miller
Oct 8, 2008·Immunological Reviews·Judith M FontanaPaul A Rota
Aug 20, 2011·Microbiology and Immunology·Takemasa SakaguchiRyoko Kawabata
Jun 9, 2006·Research in Veterinary Science·P Faísca, D Desmecht
Apr 19, 2008·The Journal of General Virology·Katrina SleemanPaul A Rota
May 9, 2009·The Journal of General Virology·Raychel Chambers, Toru Takimoto
Sep 21, 2007·Virus Research·Oliver DibbenAndrew J Easton
Apr 3, 2007·Virology·Danielle E MagoffinLin-Fa Wang
Nov 2, 2016·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Jacquelyn R BedsaulKathryn C Zoon
Jan 16, 2007·Advances in Virus Research·Andrea Paun, Paula M Pitha
May 7, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Adriana ForeroSaumendra N Sarkar

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