PMID: 11336348May 5, 2001Paper

Characterization of the amino acid transport of new immortalized choroid plexus epithelial cell lines: a novel in vitro system for investigating transport functions at the blood-cerebrospinal fluid barrier

Pharmaceutical Research
T KitazawaTetsuya Terasaki

Abstract

To establish and characterize a choroid plexus epithelial cell line (TR-CSFB) from a new type of transgenic rat harboring the temperature-sensitive simian virus 40 (ts SV 40) large T-antigen gene (Tg rat). Choroid plexus epithelial cells were isolated from the Tg rat and cultured on a collagen-coated dish at 37 degrees C during the first period of 3 days. Cells were subsequently cultured at 33 degrees C to activate large T-antigen. At the third passage, cells were cloned by colony formation and isolated from other cells using a penicillin cup. Five immortalized cell lines of choroid plexus epithelial cells (TR-CSFB 1 approximately 5) were obtained from two Tg rats. These cell lines had a polygonal cell morphology, expressed the typical choroid plexus epithelial cell marker, transthyretin, and possessed Na+, K+-ATPase on their apical side. TR-CSFBs cells expressed a large T-antigen and grew well at 33 degrees C with a doubling-time of 35 approximately 40 hr. [3H]-L-Proline uptake by TR-CSFB cells took place in an Na+-dependent, ouabain-sensitive, energy-dependent, and concentration-dependent manner. It was also inhibited by alpha-methylaminoisobutylic acid, suggesting that system A for amino acids operates in TR-CSFB cells. When...Continue Reading

Citations

Feb 18, 2005·Journal of Neurochemistry·Jiraganya BhongsatiernTetsuya Terasaki
Jan 12, 2013·Fluids and Barriers of the CNS·Tobias TenenbaumHorst Schroten
Jun 10, 2004·Biological & Pharmaceutical Bulletin·Tomohide KitanoEmi Nakashima
Oct 23, 2004·Drug Metabolism and Pharmacokinetics·Ken-ichi HosoyaTetsuya Terasaki
Nov 2, 2019·Journal of Neuroscience Methods·Ulrike ErbMichael Karremann
Apr 2, 2021·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Tetsuya Terasaki
Oct 14, 2003·Drug Discovery Today·Tetsuya TerasakiKen-ichi Hosoya
Jul 14, 2005·Progress in Neurobiology·Wolfgang Löscher, Heidrun Potschka
Dec 29, 2007·Cerebrospinal Fluid Research·Joanna Szmydynger-ChodobskaAdam Chodobski

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