PMID: 11342711May 9, 2001Paper

Characterization of the amino acids essential for the photo- and radioprotective effects of a Bowman-Birk protease inhibitor-derived nonapeptide

Protein Engineering
K H DittmannH P Rodemann

Abstract

The Bowman-Birk protease inhibitor has been reported to exert photo- and radioprotective activity. This effect was assigned to a cyclic nonapeptide sequence which is known to contain the amino acids responsible for the anti-chymotryptic activity of the BBI. The present study indicated that linearization of the nonapeptide resulted in a significant loss of anti-proteolytic activity, whereas the photo- and radioprotective capacity persisted. Substitution of the amino acids Leu or Ser of the nonapeptide, essential for the anti-proteolytic activity, with different amino acids, indicated that rather the hydrophobic features of the amino acids in this position than charge are critical to retain the photo- and radioprotective effect. These results suggest the existence of a bifunctional peptide sequence with anti-proteolytic and photo-/radioprotective capacity. However, the lack of correlation between the photo-/radioprotective activity and the anti-proteolytic activity within the peptides generated by modification of the linear nonapeptide argues for the existence of two closely colocalized domains within the nonapeptide responsible for photo-/radioprotection and protease inhibition.

References

Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P C BillingsA R Kennedy
Feb 1, 1985·International Journal of Peptide and Protein Research·Y Birk
Feb 1, 1995·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·K DittmannH P Rodemann
Jun 1, 1997·The Journal of Peptide Research : Official Journal of the American Peptide Society·T Gariani, R J Leatherbarrow
Aug 26, 1998·International Journal of Radiation Biology·K H DittmannH P Rodemann

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Citations

Sep 27, 2002·Biopolymers·Jeffrey D McBrideRobin J Leatherbarrow
Jan 14, 2003·Journal of Peptide Science : an Official Publication of the European Peptide Society·Zoltán MucsiGyörgy Orosz
Jan 30, 2007·Nature Clinical Practice. Oncology·Tanguy Y SeiwertEverett E Vokes
Feb 2, 2008·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Klaus DittmannH Peter Rodemann
Sep 27, 2014·Life Sciences in Space Research·Ann R Kennedy
Feb 18, 2021·Future Oncology·Zahra ShaghaghiSeyed Kamaledin Hadei
Jul 31, 2021·International Journal of Radiation Biology·Ann R Kennedy
Sep 12, 2006·Biomacromolecules·Manoj KumarNicole Chow

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