PMID: 11318101Apr 25, 2001Paper

Characterization of the amyR gene encoding a transcriptional activator for the amylase genes in Aspergillus nidulans

Current Genetics
Shuji TaniN Tsukagoshi

Abstract

The Aspergillus nidulans amyR gene and its cDNA were cloned and sequenced. The genomic gene comprised 2,092 bp, interrupted by two short introns, and encoded a cys-6 zinc transcriptional activator (AMYR) of 662 amino acid residues with a calculated molecular mass of 72,862 Da. Disruption of the amyR gene caused defects in the utilization of maltose and starch and abolished expression of the taaG2 gene encoding A. oryzae Taka-amylase A, which is inducibly and abundantly expressed in the wild-type A. nidulans. Expression of the amyR gene was under the control of the carbon catabolite repressor, CREA. The growth defect of the malA1 mutant on maltose was complemented by the amyR gene; and the amyR gene derived from the mutant possessed a single mutation, from A to T, at position 1,483, resulting in a substitution of His478 to Leu. These results indicate that the amyR gene is identical to the genetically defined malA gene. AMYR possessed five domains (Zn and MH1-MH4) homologous to Mal63p, a transcriptional activator for the genes involved in maltose utilization in Saccharomyces cerevisiae. The His478 to Leu substitution lay within the MH3 domain, corresponding to the negative regulatory domain of Mal63p which relieves the inhibitory...Continue Reading

Citations

Jan 19, 2012·Applied Microbiology and Biotechnology·Yuriko MurakoshiTetsuo Kobayashi
Nov 7, 2002·Current Genetics·Naoki KatoNorihiro Tsukagoshi
May 28, 2011·Journal of Industrial Microbiology & Biotechnology·Toshikazu SugimotoHiroshi Shoji
Jul 27, 2002·Journal of Biotechnology·A P MacCabeD Ramón
Mar 2, 2002·Applied and Environmental Microbiology·Naoki KatoNorihiro Tsukagoshi
Sep 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Sarah MacPhersonBernard Turcotte
Oct 13, 2006·Bioscience, Biotechnology, and Biochemistry·Takashi NakamuraTetsuo Kobayashi
Mar 8, 2007·Bioscience, Biotechnology, and Biochemistry·Tetsuo KobayashiMasayuki Machida
Sep 25, 2004·Bioscience, Biotechnology, and Biochemistry·Tatsuo ItoTetsuo Kobayashi
Feb 10, 2009·Bioscience, Biotechnology, and Biochemistry·Tomohiro MakitaTetsuo Kobayashi
Oct 8, 2011·PloS One·Jianping Sun, N Louise Glass
Dec 17, 2002·The Journal of General and Applied Microbiology·Norihiro TsukagoshiMasashi Kato
Oct 8, 2013·Bioscience, Biotechnology, and Biochemistry·Kyung-Mo SongAtsuo Kimura
Oct 31, 2003·Bioscience, Biotechnology, and Biochemistry·Kazuki NagamineKiyoshi Ito
Oct 8, 2009·Bioscience, Biotechnology, and Biochemistry·Susumu MasudaMasayuki Tanabe
Jan 5, 2011·Journal of Bioscience and Bioengineering·Jun WatanabeOsamu Akita
Oct 9, 2008·Biotechnology Advances·David Lubertozzi, Jay D Keasling
Jul 11, 2014·Fungal Genetics and Biology : FG & B·Neil Andrew BrownGustavo Henrique Goldman
Oct 22, 2013·Fungal Genetics and Biology : FG & B·Hyunjung ChungYong-Hwan Lee
Dec 28, 2002·Microbiological Research·Prasetyawan SasangkaNorihiro Tsukagoshi
Oct 24, 2009·Fungal Genetics and Biology : FG & B·Sachiko HasegawaKatsuya Gomi
Nov 25, 2017·BMC Genomics·Birgit S GrubenRonald P De Vries
Mar 14, 2007·Nature Genetics·Junhyun JeonYong-Hwan Lee
Jun 5, 2019·Bioscience, Biotechnology, and Biochemistry·Katsuya Gomi
Jun 16, 2017·Biotechnology for Biofuels·Tiziano BenocciRonald P de Vries

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