Characterization of the antigenic specificity of four different anti-(alpha 2-->8-linked polysialic acid) antibodies using lipid-conjugated oligo/polysialic acids.

The Journal of Biological Chemistry
Chihiro SatoY Inoue

Abstract

A rapid, sensitive, and facile method for screening and characterizing anti-polysialic acid (polySia) antibodies using lipid-conjugated oligo/polysialic acids (oligo/polySia) was developed, which is based on an enzyme-linked immunosorbent assay. Homooligo/polymers of alpha 2-->8-linked N-acetylneuraminic acid (Neu5Ac), N-glycoly-neuraminic acid, and 2-keto-3-deoxy-D-galacto-nononic acid (KDN) were conjugated with phosphatidylethanolamine dipalmitoyl (PE) by reductive amination to prepare neo-oligo/polysialoglycolipids (oligo/polySia-PE). Using this method, the anti-polySia equine antibody, H.46, bound to (-->8Neu5Ac alpha 2-->)n-PE, where n = 9 or more residues, a result in confirmation of previous binding studies using radiolabeled oligo/polyNeu5Ac. The antigenic specificity and sensitivity of two monoclonal anti-poly/oligoNeu5Ac antibodies (mAb.12E3 and mAb.5A5) and one anti-oligoKDN antibody (mAb.kdn8kdn), were also determined. mAb.12E3 could detect as little as 25 pg/well of oligo/polyNeu5Ac-PE, while 0.4 ng/well of oligo/polyNeu5Ac-PE to be detected. mAb.kdn8kdn detected as little as 12 ng/well of oligoKDN-PE. Using a series of oligo/polySia-PE with defined degrees of polymerization (DP), the minimum chain length for immun...Continue Reading

References

Aug 14, 1978·Biochemical and Biophysical Research Communications·S Inoue, M Iwasaki
Feb 1, 1992·Glycobiology·F A Troy
May 1, 1992·Science·J RothW J Gehring
Jul 1, 1991·The Journal of Cell Biology·A AchesonU Rutishauser
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J RothD Bitter-Suermann
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J RothJ Finne
Nov 1, 1972·The Journal of Infectious Diseases·F A WyleJ P Lowenthal
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·E R VimrF A Troy
Jan 1, 1994·Methods in Enzymology·T FeiziA M Lawson

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Citations

Apr 15, 2004·Bioorganic & Medicinal Chemistry·Toshio NishikawaMinoru Isobe
Aug 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·B QuJ Roth
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Jun 22, 2013·Journal of Biochemistry·Chihiro Sato, Ken Kitajima
Jul 7, 2009·Annual Review of Nutrition·Bing Wang
May 15, 2001·Biochemical and Biophysical Research Communications·S SzuchetK S Eaton
Nov 7, 2014·Critical Reviews in Biochemistry and Molecular Biology·Karen J ColleyChihiro Sato
Mar 10, 1998·The Journal of Comparative Neurology·D W Allan, J J Greer
Mar 10, 2007·The Journal of Comparative Neurology·Tatsunori SekiMasafumi Onodera
Apr 16, 2010·The Journal of Comparative Neurology·Shizuko MurakamiKatsuhiko Ono
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May 26, 2018·Glycobiology·Xiaoqing ZhangXue-Long Sun
May 28, 2020·Scientific Reports·Nao YamakawaChihiro Sato
Jan 26, 1996·The Journal of Biological Chemistry·J Nakayama, M Fukuda

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