Characterization of the aryl hydrocarbon receptor complex in human B lymphocytes: evidence for a distinct nuclear DNA-binding form

Archives of Biochemistry and Biophysics
S A Masten, K T Shiverick

Abstract

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) suppresses B lymphocyte proliferation and immunoglobulin production. We previously reported that the aryl hydrocarbon receptor (AhR) complex, composed of the AhR ligand binding subunit and the Ah receptor nuclear translocator (ARNT), was constitutively present in nuclear extracts from two human B lymphocyte cell lines (Biochem. Biophys. Res. Commun. 212, 27-34, 1995). The present study compared the AhR complex in the IM-9 and PJS-91 human B lymphocyte and HepG2 human hepatoma cell lines. AhR mRNA levels in the two lymphocyte cell lines were substantially lower than those in HepG2 cells, as was immunoreactive AhR protein. In contrast, ARNT mRNA and protein were expressed at a high level in all three cell lines. TCDD induction of cytochrome P450 1A1 mRNA and protein was detected in only the PJS-91 lymphocyte cell line, and at a markedly lower level than that in HepG2 cells. In gel shift assays, the cytosolic DNA-binding AhR complex in IM-9 and PJS-91 cells was indistinguishable from that in HepG2 cells. In contrast, the nuclear DNA-binding AhR complex in IM-9 and PJS-91 cells consisted of several closely migrating species, one being recognized by an AhR antibody, while an ARNT antibody r...Continue Reading

Citations

May 26, 1998·Environmental Health Perspectives·J A GrassmanG W Lucier
Jan 29, 2002·International Immunopharmacology·Nancy I Kerkvliet
Feb 27, 2003·Biochemical and Biophysical Research Communications·Patrik AnderssonAnnika Hanberg
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May 16, 2018·European Journal of Applied Physiology·Yitzhak WeinsteinGal Dubnov-Raz
Oct 24, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·B Paige LawrenceDavid L Woodland
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