PMID: 6165722Jul 10, 1981Paper

Characterization of the association of two small molecular weight RNAs with eukaryotic polysomes.

The Journal of Biological Chemistry
P W GunningP L Jeffrey

Abstract

Previous studies of the adult rat cerebral cortex have shown that two of the small molecular weight RNAs, K and L, are primarily cytoplasmic in location and are present in significant quantities in the microsomal fraction. We have examined their appearance in microsomal preparations. The results demonstrate that both K and L are, in fact, associated with polyribosomes. In particular, it is shown that (i) K and L are present in both membrane-bound and free polysomes; (ii) removal of microsomal membranes with Triton X-100 does not alter their association with microsomal polysomes; (iii) all of the K and almost one-half of the L associated with rat liver polysomes are resistant to high salt washing; and (iv) K and L are present in rabbit reticulocyte-free polysome preparations. The protein synthesis inhibitors cycloheximide and aurintricarboxylic acid significantly alter the association of K and L with rabbit reticulocyte-free polysomes. It is concluded that K and L are transiently associated with polysomes during the process of protein synthesis.

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