Characterization of the azide-dependent bacteriorhodopsin-like photocycle of salinarum halorhodopsin

Biophysical Journal
M LakatosG Váró

Abstract

The photocycle of salinarum halorhodopsin was investigated in the presence of azide. The azide binds to the halorhodopsin with 150 mM binding constant in the absence of chloride and with 250 mM binding constant in the presence of 1 M chloride. We demonstrate that the azide-binding site is different from that of chloride, and the influence of chloride on the binding constant is indirect. The analysis of the absorption kinetic signals indicates the existence of two parallel photocycles. One belongs to the 13-cis retinal containing protein and contains a single red shifted intermediate. The other photocycle, of the all-trans retinal containing halorhodopsin, resembles the cycle of bacteriorhodopsin and contains a long-living M intermediate. With time-resolved spectroscopy, the spectra of intermediates were determined. Intermediates L, N, and O were not detected. The multiexponential rise and decay of the M intermediate could be explained by the introduction of the "spectrally silent" intermediates M1, M2, and HR', HR, respectively. The electric signal measurements revealed the existence of a component equivalent with a proton motion toward the extracellular side of the membrane, which appears during the M1 to M2 transition. The di...Continue Reading

References

Sep 9, 1977·Biochemical and Biophysical Research Communications·A Matsuno-Yagi, Y Mukohata
May 28, 1979·Biochemical and Biophysical Research Communications·E V Lindley, R E MacDonald
Apr 1, 1992·Journal of Bioenergetics and Biomembranes·D OesterheltE Bamberg
Jan 1, 1986·Annual Review of Biophysics and Biophysical Chemistry·J K Lanyi
Feb 1, 1989·Trends in Biochemical Sciences·D Oesterhelt, J Tittor
Mar 1, 1985·Journal of Biochemical and Biophysical Methods·A DérJ Simon
May 1, 1995·Biophysical Journal·G VáróJ K Lanyi
Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·E BambergD Oesterhelt
May 28, 1996·Biochemistry·G VáróJ K Lanyi
Apr 2, 1998·Biochemistry·L S BrownJ K Lanyi
Jun 5, 1998·FEBS Letters·I V KalaidzidisA D Kaulen
Jun 1, 1999·FEBS Letters·Z DancsházyA Dér
Feb 2, 2000·Biophysical Journal·K LudmannG Váró
Jul 29, 2000·FEBS Letters·Z Dancsházy, Z Tokaji
Sep 14, 2000·Biochimica Et Biophysica Acta·G Váró

❮ Previous
Next ❯

Citations

Aug 7, 2002·Current Opinion in Structural Biology·Lars-Oliver Essen
Aug 2, 2003·Bioelectrochemistry·László OrosziAndrás Dér
Oct 27, 2006·The Journal of Physical Chemistry. B·Marta DorogiLaszló Nagy

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.